Overview

  • Product name

    Anti-XRCC1 (phospho S518 + T519 + T523) antibody
    See all XRCC1 primary antibodies
  • Description

    Rabbit polyclonal to XRCC1 (phospho S518 + T519 + T523)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide to a region surrounding residues Ser 518, Thr 519 and Thr 523 of Human XRCC1, Swiss Prot P18887.

  • Positive control

    • Human Controls: B-cell lymphoma, breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, metastatic lymph node, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma.
      Mouse Controls: colon carcinoma CT26, squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab195205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/1000 - 1/5000.
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.

Target

  • Function

    Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Sequence similarities

    Contains 2 BRCT domains.
  • Post-translational
    modifications

    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • Cellular localization

    Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

References

This product has been referenced in:

  • Sun C  et al. Mobile phone signal exposure triggers a hormesis-like effect in Atm(+/+) and Atm(-/-) mouse embryonic fibroblasts. Sci Rep 6:37423 (2016). WB ; Mouse . Read more (PubMed: 27857169) »
See 1 Publication for this product

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