Anti-XRCC1 (phospho S518 + T519 + T523) antibody (ab84417)
Key features and details
- Rabbit polyclonal to XRCC1 (phospho S518 + T519 + T523)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-XRCC1 (phospho S518 + T519 + T523) antibody
See all XRCC1 primary antibodies -
Description
Rabbit polyclonal to XRCC1 (phospho S518 + T519 + T523) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to Human XRCC1 (phospho S518 + T519 + T523).
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Positive control
- IHC: Human breast carcinoma, Mouse squamous cell carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsPhosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated. -
Cellular localization
Nucleus. Accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 7515 Human
- Entrez Gene: 22594 Mouse
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q60596 Mouse
- Unigene: 98493 Human
- Unigene: 4347 Mouse
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Alternative names
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
Images
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Detection of Human XRCC1 (phospho S518 + T519 + T523) by Immunohistochemistry.
Samples: FFPE serial sections of Human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for XRCC1 (phospho S518 + T519 + T523).Antibody: ab84417 used at a dilution of 1/250.
Detection: DAB staining. -
Detection of Mouse XRCC1 (phospho S518 + T519 + T523) by Immunohistochemistry.
Samples: FFPE serial sections of Mouse squamous cell carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for XRCC1 (phospho S518 + T519 + T523).Antibody: ab84417 used at a dilution of 1/250. Detection: DAB staining. -
Detection of Human XRCC1 (phospho S518 + T519 + T523) by Immunofluorescence.
Sample: FFPE section of Human breast carcinoma.Antibody: ab84417 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab84417 has been referenced in 1 publication.
- Lord T & Aitken RJ Fertilization stimulates 8-hydroxy-2'-deoxyguanosine repair and antioxidant activity to prevent mutagenesis in the embryo. Dev Biol 406:1-13 (2015). PubMed: 26234752