Key features and details
- Rabbit polyclonal to XRCC2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-XRCC2 antibody
See all XRCC2 primary antibodies
DescriptionRabbit polyclonal to XRCC2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant full length protein corresponding to Human XRCC2 aa 1-280.
MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPE GTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRL SQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDS LSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQ KASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSS NQFSLVSRCLKSNSLKKHFFIIGESGVEFC
Database link: O43543
- Extracts of HeLa, SH-SY5Y and SW480 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm, Cytoskeleton, Nucleus
- Information by UniProt
- DKFZp781P0919 antibody
- DNA repair protein XRCC2 antibody
- X ray repair complementing defective repair in Chinese hamster cells 2 antibody
All lanes : Anti-XRCC2 antibody (ab180752) at 1/500 dilution
Lane 1 : Extracts of HeLa cell line
Lane 2 : Extracts of SH-SY5Y cell line
Lane 3 : Extracts of SW480 cell line
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling XRCC2 with ab180752 at a dilution of 1/50.
Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling XRCC2 with ab180752 (shown in green) at a dilution of 1/50. Nuclear DNA was labelled with DAPI (shown in blue).
Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling XRCC2 with ab180752 (shown in green) at a dilution of 1/50.
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180752. Blue DAPI for nuclear staining.
ab180752 has been referenced in 1 publication.
- Saxena S et al. XRCC2 Regulates Replication Fork Progression during dNTP Alterations. Cell Rep 25:3273-3282.e6 (2018). PubMed: 30566856