Product nameAnti-XRCC2 antibody [EPR5149]
See all XRCC2 primary antibodies
DescriptionRabbit monoclonal [EPR5149] to XRCC2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human XRCC2 aa 200-300. The exact sequence is proprietary.
- Human testis, HeLa, SW480, SH-SY5Y, Caco 2 and Human kidney lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm, Cytoskeleton, Nucleus
- Information by UniProt
- DKFZp781P0919 antibody
- DNA repair protein XRCC2 antibody
- X ray repair complementing defective repair in Chinese hamster cells 2 antibody
All lanes : Anti-XRCC2 antibody [EPR5149] (ab124900) at 1/10000 dilution
Lane 1 : Human testis lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : SH-SY5Y lysate
Lane 5 : Caco 2 lysate
Lane 6 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
ab124900 at 1/100 dilution staining XRCC2 in permeablised HeLa cells by Flow cytometry (red). Rabbit IgG negative control antibody (green).
ab124900 has not yet been referenced specifically in any publications.