Overview

  • Product name

    Anti-XRCC2 antibody [EPR5149]
    See all XRCC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5149] to XRCC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human XRCC2 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human testis, HeLa, SW480, SH-SY5Y, Caco 2 and Human kidney lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5149
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
    • Sequence similarities

      Belongs to the RecA family. RAD51 subfamily.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm, Cytoskeleton, Nucleus
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp781P0919 antibody
      • DNA repair protein XRCC2 antibody
      • X ray repair complementing defective repair in Chinese hamster cells 2 antibody
      • X ray repair cross complementing protein 2 antibody
      • X-ray repair cross-complementing protein 2 antibody
      • Xrcc2 antibody
      • XRCC2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-XRCC2 antibody [EPR5149] (ab124900) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa lysate
      Lane 3 : SW480 lysate
      Lane 4 : SH-SY5Y lysate
      Lane 5 : Caco 2 lysate
      Lane 6 : Human kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 32 kDa

    • ab124900 at 1/100 dilution staining XRCC2 in permeablised HeLa cells by Flow cytometry (red). Rabbit IgG negative control antibody (green).

    References

    ab124900 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124900.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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