Key features and details
- Rabbit polyclonal to XRCC3
- Suitable for: ICC/IF, WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-XRCC3 antibody
See all XRCC3 primary antibodies
DescriptionRabbit polyclonal to XRCC3
Specificityab58467 detects endogenous levels of total XRCC1 protein.
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from internal sequence of human XRCC3.
- Extracts from HepG2 cells; human lung carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab58467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 38 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
- Information by UniProt
- CMM6 antibody
- DNA repair protein XRCC3 antibody
- RAD51 like antibody
All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution
Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide
Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide
Predicted band size: 38 kDa
Observed band size: 38 kDa
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
ab58467 at 1/500 dilution staining XRCC3 in COS7 cells by Immunofluorescence, in the absence or presence of the synthesized peptide.
ab58467 has not yet been referenced specifically in any publications.