Overview

  • Product name

  • Description

    Rabbit polyclonal to XRCC3
  • Host species

    Rabbit
  • Specificity

    ab58467 detects endogenous levels of total XRCC1 protein.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of human XRCC3.

  • Positive control

    • Extracts from HepG2 cells; human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab58467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function

    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • Sequence similarities

    Belongs to the RecA family. RAD51 subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

Images

  • All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide
    Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • ab58467 at 1/500 dilution staining XRCC3 in COS7 cells by Immunofluorescence, in the absence or presence of the synthesized peptide.

References

ab58467 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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