Overview

  • Product name

    Anti-XRCC3 antibody - C-terminal
    See all XRCC3 primary antibodies
  • Description

    Rabbit polyclonal to XRCC3 - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse XRCC3 aa 290-305 (C terminal).
    Sequence:

    NQLLMRLMVDRTHEDD


    Database link: Q9CXE6

  • Positive control

    • Jurkat, HT1080, COLO320 and M231 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab191088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • Function

    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • Sequence similarities

    Belongs to the RecA family. RAD51 subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of rat brain tissue staining XRCC3 with ab191088 at 1 μg/ml.

    Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • All lanes : Anti-XRCC3 antibody - C-terminal (ab191088) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : COLO320 cell lysate
    Lane 4 : M231 cell lysate

    Predicted band size: 38 kDa

References

ab191088 has not yet been referenced specifically in any publications.

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