Key features and details
- Rabbit polyclonal to XRCC4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-XRCC4 antibody
See all XRCC4 primary antibodies
DescriptionRabbit polyclonal to XRCC4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within amino acids 266-279 of Human XRCC4 (NP_071801)
- WB: HeLa cell nuclear extract. IHC-P: HBL435 xenograft tissue. IF: HeLa cell.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the XRCC4 family.
modificationsPhosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
- Information by UniProt
- DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA repair protein XRCC4 antibody
- SSMED antibody
All lanes : Anti-XRCC4 antibody (ab97351) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa cell nuclear extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 38 kDa
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft using ab97351 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling XRCC4 using ab97351 at 1/200 dilution. Lower image was also labelled with DNA probe.
ab97351 has been referenced in 4 publications.
- Deraska PV et al. NF-?B inhibition by dimethylaminoparthenolide radiosensitizes non-small-cell lung carcinoma by blocking DNA double-strand break repair. Cell Death Discov 4:10 (2018). PubMed: 29531807
- Willis NA et al. Rad51 recruitment and exclusion of non-homologous end joining during homologous recombination at a Tus/Ter mammalian replication fork barrier. PLoS Genet 14:e1007486 (2018). PubMed: 30024881
- Stelcer E et al. Chondrocytes differentiated from human induced pluripotent stem cells: Response to ionizing radiation. PLoS One 13:e0205691 (2018). PubMed: 30352062
- Willis NA et al. Mechanism of tandem duplication formation in BRCA1-mutant cells. Nature 551:590-595 (2017). PubMed: 29168504