Key features and details
- Mouse monoclonal [OTI5F3] to XRCC6BP1/KUB3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-XRCC6BP1/KUB3 antibody [OTI5F3]
See all XRCC6BP1/KUB3 primary antibodies
DescriptionMouse monoclonal [OTI5F3] to XRCC6BP1/KUB3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human XRCC6BP1/KUB3 aa 1-246. Produced in E.coli (NP_150592).
MAGAPDERRRGPAAGEQLQQQHVSCQVFPERLAQGNPQQGFFSSFFTSNQ KCQLRLLKTLETNPYVKLLLDAMKHSGCAVNKDRHFSCEDCNGNVSGGFD ASTSQIVLCQNNIHNQAHMNRVVTHELIHAFDHCRAHVDWFTNIRHLACS EVRAANLSGDCSLVNEIFRLHFGLKQHHQTCVRDRATLSILAVRNISKEV AKKAVDEVFESCFNDHEPFGRIPHNKTYARYAHRDFENRDRYYSNI
Database link: Q9Y6H3
- WB: pCMV6-ENTRY XRCC6BP1/KUB3 transfected HEK-293T cell lysate. IHC-P: Human ovary adenocarcinoma, endometrium adenocarcinoma and bladder tissue.
This product was previously labelled as XRCC6BP1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 28 kDa.|
Perform heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
RelevanceXRCC6BP1 may be involved in double-strand break repair via nonhomologous end joining.
- EC 3.4.24 antibody
- Ku70 binding protein 3 antibody
- KUB3 antibody
All lanes : Anti-XRCC6BP1/KUB3 antibody [OTI5F3] (ab236425) at 1/500 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY XRCC6BP1/KUB3 transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 28 kDa
Paraffin-embedded human ovary adenocarcinoma tissue stained for XRCC6BP1/KUB3 using ab236425 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium adenocarcinoma tissue stained for XRCC6BP1/KUB3 using ab236425 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder tissue stained for XRCC6BP1/KUB3 using ab236425 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236425 has not yet been referenced specifically in any publications.