Anti-XRCC6BP1/KUB3 antibody [OTI5F3] (ab236425)
Key features and details
- Mouse monoclonal [OTI5F3] to XRCC6BP1/KUB3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-XRCC6BP1/KUB3 antibody [OTI5F3] -
Description
Mouse monoclonal [OTI5F3] to XRCC6BP1/KUB3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human XRCC6BP1/KUB3 aa 1 to the C-terminus. Produced in E.coli (NP_150592).
Database link: Q9Y6H3 -
Positive control
- WB: pCMV6-ENTRY XRCC6BP1/KUB3 transfected HEK-293T cell lysate. IHC-P: Human ovary adenocarcinoma, endometrium adenocarcinoma and bladder tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5F3 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500. Predicted molecular weight: 28 kDa.
|
|
IHC-P |
1/150.
Perform heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes. |
Notes |
---|
WB
1/500. Predicted molecular weight: 28 kDa. |
IHC-P
1/150. Perform heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes. |
Target
-
Relevance
XRCC6BP1 may be involved in double-strand break repair via nonhomologous end joining. -
Database links
- Entrez Gene: 91419 Human
- SwissProt: Q9Y6H3 Human
-
Alternative names
- EC 3.4.24 antibody
- Ku70 binding protein 3 antibody
- KUB3 antibody
see all
Images
-
All lanes : Anti-XRCC6BP1/KUB3 antibody [OTI5F3] (ab236425) at 1/500 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY XRCC6BP1/KUB3 transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 28 kDa -
Paraffin-embedded human ovary adenocarcinoma tissue stained for XRCC6BP1/KUB3 using ab236425 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human endometrium adenocarcinoma tissue stained for XRCC6BP1/KUB3 using ab236425 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human bladder tissue stained for XRCC6BP1/KUB3 using ab236425 at 1/150 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab236425 has not yet been referenced specifically in any publications.