Key features and details
- Rabbit polyclonal to Xrn1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Xrn1 antibody
See all Xrn1 primary antibodies
DescriptionRabbit polyclonal to Xrn1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment (His-T7-tag) corresponding to Rat Xrn1 aa 1567-1723. Expressed in E.coli. N-terminal tags.
FPQPTANMMPSSSHLFGSVPWRPPVPVPGNAYHYPSYPATMPLAGGMPGG VHNQFIPLQVTKKRVANKKNFENKEAQSSQATPLQTNKPGSSEATRMTPQ EGSPASSASSQATQPVSSHVETASQGHVVSHQRSAPSSSRRKSRKLAVNF SVSKPSE
Database link: D4ABN8
- WB: Recombinant rat Xrn1 protein; MCF7 cell lysate. IHC-P: Rat stomach tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml.|
RelevanceXrn1 is the major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). May act as a tumor suppressor protein in osteogenic sarcoma (OGS). Associates with alpha and beta tubulins (By similarity). Found in a mRNP complex with RENT1, RENT2, RENT3B and XRN1.
- 5'-3' exoribonuclease 1 antibody
- Dhm2 antibody
- mXRN1 antibody
Formalin-fixed, paraffin-embedded rat stomach tissue stained for Xrn1 using ab231197 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Xrn1 antibody (ab231197) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate
Anti-Xrn1 antibody (ab231197) at 2 µg/ml + Recombinant rat Xrn1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231197 has not yet been referenced specifically in any publications.