Anti-XRN2 antibody (ab72181)
Key features and details
- Rabbit polyclonal to XRN2
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-XRN2 antibody
See all XRN2 primary antibodies -
Description
Rabbit polyclonal to XRN2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant -
Immunogen
Synthetic peptide corresponding to Human XRN2. Synthetic peptide corresponding to a region between residues 900 and 950 of human XRN2 (NP_036387.2)
Database link: Q9H0D6 -
Positive control
- WB: TCMK-1, 4T1 and CT26 whole cell lysates. IHC-P: Human colon carcinoma and mouse renal cell carcinoma. IP: HelLa
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 3 µg/mg of lysate.
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WB | (1) |
Use a concentration of 0.4 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
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IHC-P |
Use a concentration of 0.2 mg/ml.
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Notes |
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IP
Use at 3 µg/mg of lysate. |
WB
Use a concentration of 0.4 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa). |
IHC-P
Use a concentration of 0.2 mg/ml. |
Target
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Relevance
XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. -
Cellular localization
Nucleus, nucleolus. -
Database links
- Entrez Gene: 22803 Human
- Entrez Gene: 24128 Mouse
- Omim: 608851 Human
- SwissProt: Q9H0D6 Human
- SwissProt: Q9DBR1 Mouse
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Alternative names
- 5' 3' exoribonuclease 2 antibody
- DHM1 like protein antibody
- DHP protein antibody
- XRN 2 antibody
Images
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IHC image of XRN2 staining in mouse renal cell carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72181, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of XRN2 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72181, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Detection of Human XRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (3 ug per IP reaction), using ab72181 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
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All lanes : Anti-XRN2 antibody (ab72181) at 0.4 µg/ml
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : 4T1 whole cell lysate
Lane 3 : CT26 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab72181 has been referenced in 14 publications.
- Patidar PL et al. XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition. Sci Rep 10:14253 (2020). PubMed: 32859985
- Ma L et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol 38:101801 (2020). PubMed: 33232910
- Yao RW et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus. Mol Cell 76:767-783.e11 (2019). PubMed: 31540874
- Mersaoui SY et al. Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids. EMBO J 38:e100986 (2019). PubMed: 31267554
- Paschon V et al. VDAC1 is essential for neurite maintenance and the inhibition of its oligomerization protects spinal cord from demyelination and facilitates locomotor function recovery after spinal cord injury. Sci Rep 9:14063 (2019). PubMed: 31575916