• Product name

  • Description

    Rabbit polyclonal to XRN2
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 25 and 75 of human XRN2 (NP_036387.2).

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.



Our Abpromise guarantee covers the use of ab72284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/2000 - 1/10000. Detects bands of approximately 109 & 115 kDa (predicted molecular weight: 109 kDa).

    Is unsuitable for IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
    • Cellular localization

      Nucleus, nucleolus.
    • Database links

    • Alternative names

      • 5' 3' exoribonuclease 2 antibody
      • DHM1 like protein antibody
      • DHP protein antibody
      • XRN 2 antibody


    • All lanes : Anti-XRN2 antibody (ab72284) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Predicted band size: 109 kDa
      Observed band size: 109,115 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.


    This product has been referenced in:

    • Rother S  et al. NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor. FASEB J 30:174-85 (2016). Read more (PubMed: 26340924) »
    • Ternette N  et al. Label-free quantitative proteomics reveals regulation of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and 5'-3'-exoribonuclease 2 (XRN2) during respiratory syncytial virus infection. Virol J 8:442 (2011). WB ; Human . Read more (PubMed: 21933386) »
    See all 2 Publications for this product

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