Overview

  • Product name

  • Description

    Rabbit polyclonal to XRN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human XRN2 (NP_036387.2)

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/2000 - 1/10000. Detects bands of approximately 109 and 117 kDa (predicted molecular weight: 109 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
    • Cellular localization

      Nucleus, nucleolus.
    • Database links

    • Alternative names

      • 5' 3' exoribonuclease 2 antibody
      • DHM1 like protein antibody
      • DHP protein antibody
      • XRN 2 antibody

    Images

    • All lanes : Anti-XRN2 antibody (ab72307) at 0.04 µg/ml

      Lane 1 : Whole cell
      lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell
      lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell
      lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell
      lysate from 293T cells at 50 µg
      Lane 5 : Whole cell
      lysate from NIH 3T3 cells at 50 µg

      Predicted band size: 109 kDa
      Observed band size: 109,117 kDa
      why is the actual band size different from the predicted?

    References

    ab72307 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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