XTT Assay Kit (ab232856)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
XTT Assay Kit
See all Cell viability/proliferation kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Product overview
XTT Assay Kit ab232856 provides an easy to use tool for use with cell cultures to study changes in the number of cells and their metabolic activity. This provides insights into cell viability, cell proliferation, and cytotoxicity.
The XTT assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.
Reduction of XTT during the assay produces a water-soluble orange-colored formazan product which dissolves directly into the culture medium, eliminating the need for an additional solubilization step (as is required in the MTT assay, but not in the MTS assay).
The formazan product can be detected using an absorbance-based microplate reader.
As the reagents used in the XTT and their products are non-toxic and the assay does not involve cell lysis, the XTT assay can be used to measure multiple timepoints or for continual measurement.
XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) is a tetrazolium-based compound like the reagents used in the MTT assay and the MTS assay.
Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests Electron Mediator Solution 1 x 600µl XTT Developer Reagent 1 x 600µl -
Research areas
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Relevance
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
Alternative names
- Cell Cytotoxicity
- cell tracking
Images
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A typical cell titration experiment using HL-60 target cells and XTT assay kit ab232856A typical cell titration experiment using HL-60 target cells and XTT assay kit ab232856.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab232856 has been referenced in 4 publications.
- Bijle MN et al. Combined effect of arginine and fluoride on the growth of Lactobacillus rhamnosus GG. Sci Rep 11:973 (2021). PubMed: 33441658
- Wang X et al. Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-?Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes. Int J Nanomedicine 15:3483-3496 (2020). PubMed: 32523344
- Zeineh N et al. Efficaciousness of Low Affinity Compared to High Affinity TSPO Ligands in the Inhibition of Hypoxic Mitochondrial Cellular Damage Induced by Cobalt Chloride in Human Lung H1299 Cells. Biomedicines 8:N/A (2020). PubMed: 32370132
- Duforestel M et al. Glyphosate Primes Mammary Cells for Tumorigenesis by Reprogramming the Epigenome in a TET3-Dependent Manner. Front Genet 10:885 (2019). PubMed: 31611907