Product nameXTT sodium salt, Tetrazolium salt
DescriptionTetrazolium salt for use in colorimetric assays for cell viability and proliferation
- 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
- sodium 3'-[1-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6- nitro)benzene-sulfonic acid
Tetrazolium sodium salt for use in colorimetric assays for cell viability and proliferation. Cleavage of XTT by dehydrogenase enzymes of metabolically active cells yields a highly colored, water soluble formazan product, an advantage over the use of MTT due to MTT requiring a formazan crystal solubilization step. Has been used to asses valvular injury for testing cryopreserved cardiac valves.
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 2.5 mg/ml (with heating)
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Perumal P et al. Role for cell density in antifungal drug resistance in Candida albicans biofilms. Antimicrob Agents Chemother 51:2454-63 (2007). Read more (PubMed: 17502416) »
- Shimamura T et al. Relationship between the reduction of tetrazolium salt XTT and DNA strand breakage with aminosugars. J Agric Food Chem 48:1204-9 (2000). Read more (PubMed: 10775373) »
- Lu JH et al. XTT-colorimetric assay as a marker of viability in cryoprocessed cardiac valve. J Mol Cell Cardiol 29:1189-94 (1997). Read more (PubMed: 9160870) »
- Roehm NW et al. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J Immunol Methods 142:257-65 (1991). Read more (PubMed: 1919029) »