Anti-XYLT1 antibody (ab224240)
Key features and details
- Rabbit polyclonal to XYLT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XYLT1 antibody
See all XYLT1 primary antibodies -
Description
Rabbit polyclonal to XYLT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Chimpanzee -
Immunogen
Recombinant fragment corresponding to Human XYLT1 aa 800-950.
Database link: Q86Y38 -
Positive control
- IHC-P: Human skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. -
Tissue specificity
Widely expressed. Expressed at higher level in placenta, kidney and pancreas. Weakly expressed in skeletal muscle. -
Pathway
Glycan metabolism; chondroitin sulfate biosynthesis.
Glycan metabolism; heparan sulfate biosynthesis. -
Sequence similarities
Belongs to the glycosyltransferase 14 family. XylT subfamily. -
Post-translational
modificationsContains 7 disulfide bonds.
N-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Secreted. Some fraction is also found in the extracellular space together with chondroitin sulfate proteoglycans, suggesting that it can be secreted. - Information by UniProt
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Database links
- Entrez Gene: 453955 Chimpanzee
- Entrez Gene: 494008 Dog
- Entrez Gene: 64131 Human
- Entrez Gene: 233781 Mouse
- Entrez Gene: 64133 Rat
- Omim: 608124 Human
- SwissProt: Q5QQ57 Chimpanzee
- SwissProt: Q5QQ56 Dog
see all -
Alternative names
- Beta D xylosyltransferase 1 antibody
- O xylosyltransferase 1 antibody
- Peptide O-xylosyltransferase 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224240 has not yet been referenced specifically in any publications.