Anti-XYLT2 antibody (ab155193)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-XYLT2 antibody
See all XYLT2 primary antibodies -
DescriptionRabbit polyclonal to XYLT2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
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Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 272-520 of Human XYLT2 (Uniprot ID: Q9H1B5).
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Positive control
- 293T, A431, HeLa, Raji whole cell lysate; HeLa cells
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General notesStore as a concentrated solution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 97 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionProbably catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes (By similarity). Its enzyme activity has not been demonstrated.
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Tissue specificityWidely expressed. Expressed at higher level in kidney and pancreas.
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PathwayGlycan metabolism; chondroitin sulfate biosynthesis.
Glycan metabolism; heparan sulfate biosynthesis. -
Sequence similaritiesBelongs to the glycosyltransferase 14 family. XylT subfamily.
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Post-translational
modificationsContains disulfide bonds. -
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
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Database links
- Entrez Gene: 64132 Human
- Entrez Gene: 217119 Mouse
- Entrez Gene: 64134 Rat
- Omim: 608125 Human
- SwissProt: Q5QQ50 Dog
- SwissProt: Q9H1B5 Human
- SwissProt: Q9EPL0 Mouse
- SwissProt: Q9EPI0 Rat
see all -
Alternative names
- Peptide O xylosyltransferase 1 antibody
- Peptide O-xylosyltransferase 1 antibody
- protein xylosyltransferase 2 antibody
see all
Images
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Western blot - Anti-XYLT2 antibody (ab155193)Anti-XYLT2 antibody (ab155193) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 97 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-XYLT2 antibody (ab155193)Immunofluorescent analysis of methanol-fixed HeLa cells labeling XYLT2 with ab155193 at 1/500 dilution (green). Alpha tubulin filaments were labeled red.
References
ab155193 has not yet been referenced specifically in any publications.
