Anti-XYLT2 antibody (ab155193)
Key features and details
- Rabbit polyclonal to XYLT2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XYLT2 antibody -
Description
Rabbit polyclonal to XYLT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 272-520 of Human XYLT2 (Uniprot ID: Q9H1B5).
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Positive control
- 293T, A431, HeLa, Raji whole cell lysate; HeLa cells
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General notes
Store as a concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Chicken
Cow
Dog
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 97 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 97 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Probably catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes (By similarity). Its enzyme activity has not been demonstrated. -
Tissue specificity
Widely expressed. Expressed at higher level in kidney and pancreas. -
Pathway
Glycan metabolism; chondroitin sulfate biosynthesis.
Glycan metabolism; heparan sulfate biosynthesis. -
Sequence similarities
Belongs to the glycosyltransferase 14 family. XylT subfamily. -
Post-translational
modificationsContains disulfide bonds. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 64132 Human
- Entrez Gene: 217119 Mouse
- Entrez Gene: 64134 Rat
- Omim: 608125 Human
- SwissProt: Q5QQ50 Dog
- SwissProt: Q9H1B5 Human
- SwissProt: Q9EPL0 Mouse
- SwissProt: Q9EPI0 Rat
see all -
Alternative names
- Peptide O xylosyltransferase 1 antibody
- Peptide O-xylosyltransferase 1 antibody
- protein xylosyltransferase 2 antibody
see all
Images
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Western blot - Anti-XYLT2 antibody (ab155193)Anti-XYLT2 antibody (ab155193) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 97 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-XYLT2 antibody (ab155193)Immunofluorescent analysis of methanol-fixed HeLa cells labeling XYLT2 with ab155193 at 1/500 dilution (green). Alpha tubulin filaments were labeled red.
Protocols
References (0)
ab155193 has not yet been referenced specifically in any publications.
