• Product name

    Anti-Y14 antibody [EPR13945(B)]
    See all Y14 primary antibodies
  • Description

    Rabbit monoclonal [EPR13945(B)] to Y14
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Y14 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y5S9

  • Positive control

    • HeLa, Jurkat, HepG2 and 293T cell lysates. Permeabilized Jurkat cells. Immunoprecipitation pellet from Jurkat whole cell lysate (ab7899).
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab181038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.


  • Function

    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.
  • Tissue specificity

  • Sequence similarities

    Belongs to the RBM8A family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Binder of OVCA1 1 antibody
    • Binder of OVCA1-1 antibody
    • BOV 1 antibody
    • BOV 1A antibody
    • BOV 1B antibody
    • BOV 1C antibody
    • BOV-1 antibody
    • BOV1 antibody
    • BOV1A antibody
    • BOV1B antibody
    • BOV1C antibody
    • HSPC 114 antibody
    • HSPC114 antibody
    • MDS 014 antibody
    • MDS014 antibody
    • RBM 8 antibody
    • RBM 8A antibody
    • RBM 8B antibody
    • RBM8 antibody
    • rbm8a antibody
    • RBM8A_HUMAN antibody
    • RBM8B antibody
    • Ribonucleoprotein RBM 8 antibody
    • Ribonucleoprotein RBM 8A antibody
    • Ribonucleoprotein RBM8 antibody
    • Ribonucleoprotein RBM8A antibody
    • RNA binding motif protein 8 antibody
    • RNA binding motif protein 8A antibody
    • RNA binding motif protein 8B antibody
    • RNA binding protein 8A antibody
    • RNA binding protein Y14 antibody
    • RNA-binding motif protein 8A antibody
    • RNA-binding protein 8A antibody
    • RNA-binding protein Y14 antibody
    • ZNRP antibody
    • ZRNP 1 antibody
    • ZRNP1 antibody
    see all


  • All lanes : Anti-Y14 antibody [EPR13945(B)] (ab181038) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 19 kDa

  • Flow cytometric analysis of permeabilized Jurkat cells using ab181038 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) Jurkat cell lysate or (Lane 2) 1XPBS (negative control) using ab181038 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.


This product has been referenced in:

  • Shadle SC  et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet 13:e1006658 (2017). Read more (PubMed: 28273136) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (fibroblast)
Yes - 0.3% Triton X-100 in blocking buffer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 12 2018

Western blot
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 11 2016

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