Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11020] to YAF2
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-YAF2 antibody [EPR11020]
See all YAF2 primary antibodies
DescriptionRabbit monoclonal [EPR11020] to YAF2
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
within Human YAF2 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IY57
- Human skeletal muscle and fetal heart lysates.
This product was previously labelled as YY1 associated factor 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 20 kDa.|
|IP||1/10 - 1/100.|
FunctionBinds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling.
Sequence similaritiesContains 1 RanBP2-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- MGC41856 antibody
- YAF2 antibody
- YAF2_HUMAN antibody
- YY1-associated factor 2 antibody
All lanes : Anti-YAF2 antibody [EPR11020] (ab177945) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa
Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1X PBS (negative control) using ab177945 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab177945 has not yet been referenced specifically in any publications.