Recombinant Anti-YAF2 antibody [EPR11020] (ab177945)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-YAF2 antibody [EPR11020]
See all YAF2 primary antibodies -
Description
Rabbit monoclonal [EPR11020] to YAF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
within Human YAF2 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IY57 -
Positive control
- Human skeletal muscle and fetal heart lysates.
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General notes
This product was previously labelled as YY1 associated factor 2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11020 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 20 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling. -
Sequence similarities
Contains 1 RanBP2-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10138 Human
- Entrez Gene: 67057 Mouse
- Entrez Gene: 690262 Rat
- Omim: 607534 Human
- SwissProt: Q8IY57 Human
- SwissProt: Q99LW6 Mouse
- Unigene: 649195 Human
- Unigene: 708084 Human
see all -
Alternative names
- MGC41856 antibody
- YAF2 antibody
- YAF2_HUMAN antibody
- YY1-associated factor 2 antibody
Images
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![Western blot - Anti-YAF2 antibody [EPR11020] (ab177945)](https://www.abcam.com/ps/products/177/ab177945/Images/ab177945-205934-anti-yy1-associated-factor-2-antibody-epr11020-western-blot.jpg)
Western blot - Anti-YAF2 antibody [EPR11020] (ab177945)All lanes : Anti-YAF2 antibody [EPR11020] (ab177945) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa -
![Immunoprecipitation - Anti-YAF2 antibody [EPR11020] (ab177945)](https://www.abcam.com/ps/products/177/ab177945/Images/ab177945-205935-anti-yy1-associated-factor-2-antibody-epr11020-immunoprecipitation.jpg)
Immunoprecipitation - Anti-YAF2 antibody [EPR11020] (ab177945)Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1X PBS (negative control) using ab177945 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Datasheets and documents
References
ab177945 has not yet been referenced specifically in any publications.
