Anti-YAP1 antibody (ab226817)
Key features and details
- Rabbit polyclonal to YAP1
- Suitable for: WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-YAP1 antibody
See all YAP1 primary antibodies -
Description
Rabbit polyclonal to YAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Chicken
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Immunogen
Synthetic peptide within Human YAP1 (N terminal). The exact sequence is proprietary.
Database link: P46937 -
Positive control
- WB: A431, HeLa and HepG2 cell lysate; zebrafish brain and eye tissue lysate. IP: HeLa cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 54 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). -
Tissue specificity
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). -
Sequence similarities
Belongs to the YORKIE family.
Contains 2 WW domains. -
Post-translational
modificationsPhosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. -
Cellular localization
Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. - Information by UniProt
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Database links
- Entrez Gene: 396171 Chicken
- Entrez Gene: 10413 Human
- Entrez Gene: 561411 Zebrafish
- Omim: 606608 Human
- SwissProt: P46936 Chicken
- SwissProt: P46937 Human
- SwissProt: Q1L8J7 Zebrafish
- Unigene: 503692 Human
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Alternative names
- 65 kDa Yes associated protein antibody
- 65 kDa Yes-associated protein antibody
- COB1 antibody
see all
Images
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Western blot - Anti-YAP1 antibody (ab226817)All lanes : Anti-YAP1 antibody (ab226817) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa transfected with YAP1 shRNA cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa -
Western blot - Anti-YAP1 antibody (ab226817)All lanes : Anti-YAP1 antibody (ab226817) at 1/1000 dilution
Lane 1 : Zebrafish brain tissue lysate
Lane 2 : Zebrafish eye tissue lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa -
Immunoprecipitation - Anti-YAP1 antibody (ab226817)YAP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab226817. Western blot was performed from the immunoprecipitate using ab226817 at 1/100 dilution.
Lane 1: Rabbit IgG instead of ab226817 in HeLa whole cell lysate.
Lane 2: ab226817 IP in HeLa whole cell lysate. -
Western blot - Anti-YAP1 antibody (ab226817)All lanes : Anti-YAP1 antibody (ab226817) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab226817 has not yet been referenced specifically in any publications.
