Overview

  • Product name

  • Description

    Rabbit polyclonal to YAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human YAP1 aa 350-400. The exact sequence is proprietary. (NP_006097.1).
    Database link: P46937-3

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab246007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
    • Tissue specificity

      Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
    • Sequence similarities

      Belongs to the YORKIE family.
      Contains 2 WW domains.
    • Post-translational
      modifications

      Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
    • Cellular localization

      Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
    • Information by UniProt
    • Database links

    • Alternative names

      • 65 kDa Yes associated protein antibody
      • 65 kDa Yes-associated protein antibody
      • COB1 antibody
      • YAp 1 antibody
      • YAP 65 antibody
      • YAP antibody
      • YAP-1 antibody
      • YAP1 antibody
      • YAP1_HUMAN antibody
      • YAP2 antibody
      • YAP65 antibody
      • yes -associated protein delta antibody
      • Yes associated protein 1 65kDa antibody
      • Yes associated protein 1 antibody
      • Yes associated protein 2 antibody
      • yes associated protein beta antibody
      • YKI antibody
      • Yorkie homolog antibody
      see all

    Images

    • YAP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab246007 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-YAP1 1 antibody, which recognizes a upstream epitope at 1 µg/ml.

      Lane 1: ab246007 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for YAP1 using ab246007 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    References

    ab246007 has not yet been referenced specifically in any publications.

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