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    yb1-antibody-ep2706y-ab76540.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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RabMAb

Anti-YB1 antibody [EP2706Y] (ab76540)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Flow Cytometry - Anti-YB1 antibody [EP2706Y] (ab76540)
  • Western blot - Anti-YB1 antibody [EP2706Y] (ab76540)

Key features and details

  • Rabbit monoclonal [EP2706Y] to YB1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human YB1 protein (ab187443)
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Overview

  • Product name

    Anti-YB1 antibody [EP2706Y]
    See all YB1 primary antibodies
  • Description

    Rabbit monoclonal [EP2706Y] to YB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human YB1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat and MCF7 cell lysates; permeabilized HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2706Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Jurkat cytoplasmic extract lysate (ab14842)
    • MCF7 nuclear extract lysate (ab14860)
  • Recombinant Protein

    • Recombinant Human YB1 protein (ab187443)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/20000. Predicted molecular weight: 36 kDa.
Flow Cyt
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/10000 - 1/20000. Predicted molecular weight: 36 kDa.
Flow Cyt
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
      The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
    • Sequence similarities

      Contains 1 CSD (cold-shock) domain.
    • Post-translational
      modifications

      Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
      In the absence of phosphorylation the protein is retained in the cytoplasm.
      Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
    • Target information above from: UniProt accession P67809 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4904 Human
      • Omim: 154030 Human
      • SwissProt: P67809 Human
      • Unigene: 473583 Human
      • Alternative names

        • BP 8 antibody
        • CBF-A antibody
        • CCAAT binding transcription factor I subunit A antibody
        • CCAAT-binding transcription factor I subunit A antibody
        • CSDA2 antibody
        • CSDB antibody
        • DBPB antibody
        • DNA binding protein B antibody
        • DNA-binding protein B antibody
        • EFI-A antibody
        • Enhancer factor I subunit A antibody
        • MDR NF1 antibody
        • MGC104858 antibody
        • MGC110976 antibody
        • MGC117250 antibody
        • NSEP 1 antibody
        • NSEP1 antibody
        • Nuclease sensitive element binding protein 1 antibody
        • Nuclease-sensitive element-binding protein 1 antibody
        • p50 antibody
        • Q15905 antibody
        • Y-box binding protein 1 antibody
        • Y-box transcription factor antibody
        • Y-box-binding protein 1 antibody
        • YB 1 antibody
        • YB-1 antibody
        • YBOX1_HUMAN antibody
        • YBX 1 antibody
        • ybx1 antibody
        see all

      Images

      • Flow Cytometry - Anti-YB1 antibody [EP2706Y] (ab76540)
        Flow Cytometry - Anti-YB1 antibody [EP2706Y] (ab76540)
        Flow cytometric analysis of permeabilized HeLa cells using ab76540 at 1/60 dilution (red) or a rabbit IgG (negative) (green)
      • Western blot - Anti-YB1 antibody [EP2706Y] (ab76540)
        Western blot - Anti-YB1 antibody [EP2706Y] (ab76540)
        All lanes : Anti-YB1 antibody [EP2706Y] (ab76540) at 1/20000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP at 1/1000 dilution

        Predicted band size: 36 kDa
        Observed band size: 47 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab76540? Please let us know so that we can cite the reference in this datasheet.

      ab76540 has been referenced in 4 publications.

      • Weydert C  et al. Y-box-binding protein 1 supports the early and late steps of HIV replication. PLoS One 13:e0200080 (2018). PubMed: 29995936
      • Kariminia A  et al. Y-box-binding protein 1 contributes to IL-7-mediated survival signaling in B-cell precursor acute lymphoblastic leukemia. Oncol Lett 13:497-505 (2017). PubMed: 28123588
      • Davies AH  et al. YB-1 transforms human mammary epithelial cells through chromatin remodeling leading to the development of basal-like breast cancer. Stem Cells 32:1437-50 (2014). PubMed: 24648416
      • Pu L  et al. Development of a Chemiluminescence Immunoassay for Serum YB-1 and its Clinical Application as a Potential Diagnostic Marker for Hepatocellular Carcinoma. Hepat Mon 13:e8918 (2013). PubMed: 24069038

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-YB1 antibody [EP2706Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (293T)
      Loading amount
      30000 cells
      Specification
      293T
      Gel Running Conditions
      Reduced Denaturing (4-20% TrisGlycine)
      Blocking step
      5% Milk, 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 24 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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