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  1. Link

    yb1-antibody-ep2708y-ab76149.pdf

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RecombinantRabMAb

Recombinant Anti-YB1 antibody [EP2708Y] (ab76149)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (49)

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Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)
  • Anti-YB1 antibody [EP2708Y] (ab76149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2708Y] to YB1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

You may also be interested in

Primary
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Anti-Nucleolin antibody [EPR7951] (ab134164)
Protein
Product image
Recombinant Human YB1 protein (ab187443)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-YB1 antibody [EP2708Y]
    See all YB1 primary antibodies
  • Description

    Rabbit monoclonal [EP2708Y] to YB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human YB1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab175051)

  • Positive control

    • WB: HeLa, SW480, A549, C6 PC-12, NIH/3T3, Raw264.7 and MCF7 cell lysates. IHC-P: Human kidney, human cervical carcinoma, mouse liver and rat stomach tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HEK293, HeLa and MCF-7 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2708Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Alternative Versions

    • HRP Anti-YB1 antibody [EP2708Y] (ab204014)
    • Anti-YB1 antibody [EP2708Y] - BSA and Azide free (ab239875)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • SW480 whole cell lysate (ab3957)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • HEK-293 whole cell lysate (ab7902)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human YB1 protein (ab187443)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (2)
1/100.

For unpurified use at 5µg/ml.

WB (1)
1/1000. Predicted molecular weight: 36 kDa.

For unpurified use at 1/10000 - 1/20000.

IP
1/30.
IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
Flow Cyt (Intra)
1/50 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.

For unpurified use at 5µg/ml.

WB
1/1000. Predicted molecular weight: 36 kDa.

For unpurified use at 1/10000 - 1/20000.

IP
1/30.
IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
  • Sequence similarities

    Contains 1 CSD (cold-shock) domain.
  • Post-translational
    modifications

    Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
    In the absence of phosphorylation the protein is retained in the cytoplasm.
    Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
  • Target information above from: UniProt accession P67809 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4904 Human
    • Entrez Gene: 22608 Mouse
    • Entrez Gene: 500538 Rat
    • Omim: 154030 Human
    • SwissProt: P67809 Human
    • SwissProt: P62960 Mouse
    • SwissProt: P62961 Rat
    • Unigene: 473583 Human
    • Unigene: 258204 Mouse
    • Unigene: 110976 Rat
    • Unigene: 194909 Rat
    see all
  • Alternative names

    • BP 8 antibody
    • CBF-A antibody
    • CCAAT binding transcription factor I subunit A antibody
    • CCAAT-binding transcription factor I subunit A antibody
    • CSDA2 antibody
    • CSDB antibody
    • DBPB antibody
    • DNA binding protein B antibody
    • DNA-binding protein B antibody
    • EFI-A antibody
    • Enhancer factor I subunit A antibody
    • MDR NF1 antibody
    • MGC104858 antibody
    • MGC110976 antibody
    • MGC117250 antibody
    • NSEP 1 antibody
    • NSEP1 antibody
    • Nuclease sensitive element binding protein 1 antibody
    • Nuclease-sensitive element-binding protein 1 antibody
    • p50 antibody
    • Q15905 antibody
    • Y-box binding protein 1 antibody
    • Y-box transcription factor antibody
    • Y-box-binding protein 1 antibody
    • YB 1 antibody
    • YB-1 antibody
    • YBOX1_HUMAN antibody
    • YBX 1 antibody
    • ybx1 antibody
    see all

Images

  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    All lanes : Anti-YB1 antibody [EP2708Y] (ab76149) at 1/1000 dilution (purified)

    Lane 1 : NIH/3T3 whole cell lysate
    Lane 2 : Raw264.7 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    All lanes : Anti-YB1 antibody [EP2708Y] (ab76149) at 1/10000 dilution (purified)

    Lane 1 : C6 whole cell lysate
    Lane 2 : PC-12 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    All lanes : Anti-YB1 antibody [EP2708Y] (ab76149) at 1/1000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : SW480 whole cell lysate
    Lane 3 : A549 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling YB1 with purified ab76149 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling YB1 with purified ab76149 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YB1 with purified ab76149 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EP2708Y] (ab76149)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YB1 with purified ab76149 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (ab76149)
    Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (ab76149)

    Intracellular Flow Cytometry analysis of HeLa cells labelling YB1 with purified ab76149 at 1/90 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)

    ab76149 (purified) at 1/30 immunoprecipitating YB1 in MCF-7 whole cell lysate.

    Lane 1 (input): MCF-7 whole cell lysate (10µg)

    Lane 2 (+): ab76149 + MCF-7 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76149 in MCF-7 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)

    ab76149 (purified) at 1/30 immunoprecipitating YB1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab76149 + HeLa whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76149 in HeLa whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    Western blot - Anti-YB1 antibody [EP2708Y] (ab76149)
    All lanes : Anti-YB1 antibody [EP2708Y] (ab76149) at 1/200000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EP2708Y] (ab76149)

    ICC/IF image of unpurified ab76149 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab76149, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (ab76149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling YB1 with unpurified ab76149 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (ab76149)
    Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (ab76149)

    Overlay histogram showing HeLa cells stained with unpurified ab76149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab76149, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions. 

     

     

  • Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)
    Immunoprecipitation - Anti-YB1 antibody [EP2708Y] (ab76149)

    YB1 was immunoprecipitated using 0.5mg HEK293 whole cell extract, 10µg of Rabbit monoclonal to YB1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HEK293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with unpurified ab76149.
    Secondary: Mouse monoclonal [SB62a] secondary antibody to rabbit IgG light chain (HRP) (ab99697).
    Band: 46kDa: YB1.

  • Anti-YB1 antibody [EP2708Y] (ab76149)
    Anti-YB1 antibody [EP2708Y] (ab76149)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (49)

Publishing research using ab76149? Please let us know so that we can cite the reference in this datasheet.

ab76149 has been referenced in 49 publications.

  • Gu Y  et al. The cancer-testis gene, MEIOB, sensitizes triple-negative breast cancer to PARP1 inhibitors by inducing homologous recombination deficiency. Cancer Biol Med 18:74-87 (2021). PubMed: 33628586
  • Tanaka T  et al. 7-Hydorxyindirubin is capable of specifically inhibiting anticancer drug-induced YB-1 nuclear translocation without showing cytotoxicity in HepG2 hepatocellular carcinoma cells. Biochem Biophys Res Commun 544:15-21 (2021). PubMed: 33516877
  • Zhu H  et al. Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay. Sci Adv 7:N/A (2021). PubMed: 33627425
  • Ban Y  et al. RNA-binding protein YBX1 promotes cell proliferation and invasiveness of nasopharyngeal carcinoma cells via binding to AURKA mRNA. J Cancer 12:3315-3324 (2021). PubMed: 33976741
  • Wang W  et al. Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation. Onco Targets Ther 14:1007-1020 (2021). PubMed: 33603407
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-YB1 antibody [EP2708Y]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (whole embryo 3.5-4.5 hours past fertilization)
Permeabilization
Yes - -20°C MeOH storage over night
Specification
whole embryo 3.5-4.5 hours past fertilization
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 15 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-YB1 antibody [EP2708Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 17 2017

Western blot abreview for Anti-YB1 antibody [EP2708Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Renal proximal tubular epithelial cells)
Loading amount
10 µg
Specification
Renal proximal tubular epithelial cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Dr. Robert Jenkins

Verified customer

Submitted Apr 20 2009

Question

Would you please provide me with the antibody concentrations for the following products? 1) ab92547 (anti-vimentin) Lot#GR27460-8 2) ab76149 (anti-YB-1) Lot#GR35413-5

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Abcam community

Verified customer

Asked on Nov 28 2012

Answer

Thank you for your enquiry. I can confirm that these products are sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant generally will not have a concentration stated on the datasheet because the presence of additional proteins within the product may interfere with quantification. However I am happy to say that our Epitomics group does provide concentrations for many of their RabMAb tissue culture supernatant antibodies. So I can provide the following information to you: I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Abcam Scientific Support

Answered on Nov 28 2012

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