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    yb1-peptide-ab12411.pdf

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YB1 peptide (ab12411)

  • Datasheet
  • SDS
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Western blot - YB1 peptide (ab12411)

    Key features and details

    • Suitable for: Blocking

    Description

    • Product name

      YB1 peptide
      See all YB1 proteins and peptides
    • Animal free

      No
    • Nature

      Synthetic

      Associated products

      • Corresponding Antibody

        • Anti-YB1 antibody (ab12148)

      Specifications

      Our Abpromise guarantee covers the use of ab12411 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Blocking - Blocking peptide for Anti-YB1 antibody (ab12148)

      • Form

        Liquid
      • Additional notes

        - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
        - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
        - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
        - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
        - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Information available upon request.

      General Info

      • Alternative names

        • BP 8
        • CBF-A
        • CCAAT binding transcription factor I subunit A
        • CCAAT-binding transcription factor I subunit A
        • CSDA2
        • CSDB
        • DBPB
        • DNA binding protein B
        • DNA-binding protein B
        • EFI-A
        • Enhancer factor I subunit A
        • MDR NF1
        • MGC104858
        • MGC110976
        • MGC117250
        • NSEP 1
        • NSEP1
        • Nuclease sensitive element binding protein 1
        • Nuclease-sensitive element-binding protein 1
        • p50
        • Q15905
        • Y-box binding protein 1
        • Y-box transcription factor
        • Y-box-binding protein 1
        • YB 1
        • YB-1
        • YBOX1_HUMAN
        • YBX 1
        • ybx1
        see all
      • Function

        Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
        The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
      • Sequence similarities

        Contains 1 CSD (cold-shock) domain.
      • Post-translational
        modifications

        Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
        In the absence of phosphorylation the protein is retained in the cytoplasm.
        Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
      • Cellular localization

        Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
      • Target information above from: UniProt accession P67809 The UniProt Consortium
        The Universal Protein Resource (UniProt) in 2010
        Nucleic Acids Res. 38:D142-D148 (2010) .

        Information by UniProt

      Images

      • Western blot - YB1 peptide (ab12411)
        Western blot - YB1 peptide (ab12411)

        Lanes 1 to 10: Ab12148 - Rabbit polyclonal to YB1 (1.4µg/ml)     

        Lanes 1 to 10: 20µg of cell lysate per lane
        Lanes 6 to 10: blocking peptide used at 1µg/ml

        Lane 1: HeLa Nuclear Extract
        Lane 2: HeLa Whole Cell Lysate
        Lane 3: MCF-7 Cell Lysate
        Lane 4: Jurkat Whole Cell Lysate
        Lane 5: HEK293 Whole Cell Lysate
        Lane 6: HeLa Nuclear Extract + YB1 blocking peptide (ab12411)
        Lane 7: HeLa Whole Cell Lysate +  YB1 blocking peptide (ab12411)
        Lane 8: MCF-7 Cell Lysate +  YB1 blocking peptide (ab12411)
        Lane 9: Jurkat Whole Cell Lysate +  YB1 blocking peptide (ab12411)
        Lane 10: HEK293 Whole Cell Lysate +  YB1 blocking peptide (ab12411)

        Expected MW: 36 kDa

        A band of approx 36 kDa was partially blocked in several cell lines using a YB1 blo

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab12411? Please let us know so that we can cite the reference in this datasheet.

      ab12411 has been referenced in 1 publication.

      • Heumann A  et al. Up regulation and nuclear translocation of Y-box binding protein 1 (YB-1) is linked to poor prognosis in ERG-negative prostate cancer. Sci Rep 7:2056 (2017). PubMed: 28515422

      Customer reviews and Q&As

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