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    yb1-phospho-s102-antibody-ab138654.pdf

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Anti-YB1 (phospho S102) antibody (ab138654)

  • Datasheet
  • SDS
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Western blot - Anti-YB1 (phospho S102) antibody (ab138654)

    Key features and details

    • Rabbit polyclonal to YB1 (phospho S102)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human YB1 protein (ab187443)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-YB1 (phospho S102) antibody
      See all YB1 primary antibodies
    • Description

      Rabbit polyclonal to YB1 (phospho S102)
    • Host species

      Rabbit
    • Specificity

      ab138654 detects endogenous levels of YB1 only when phosphorylated at S102
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Synthesized phospho-peptide derived from an internal region of Human YB1 around the phosphorylation site of S102.

    • Positive control

      • Extracts from HepG2 cells treated with PMA.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab138654 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Stem Cells
      • Embryonic Stem Cells
      • Intracellular
      • Epigenetics and Nuclear Signaling
      • Chromatin Binding Proteins
      • DNA / RNA binding
      • Developmental Biology
      • Embryogenesis
      • Embryonic stem cells
      • Surface molecules

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human YB1 protein (ab187443)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab138654 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 36 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 36 kDa.

    Target

    • Function

      Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
      The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
    • Sequence similarities

      Contains 1 CSD (cold-shock) domain.
    • Post-translational
      modifications

      Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
      In the absence of phosphorylation the protein is retained in the cytoplasm.
      Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
    • Target information above from: UniProt accession P67809 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4904 Human
      • Entrez Gene: 22608 Mouse
      • Entrez Gene: 500538 Rat
      • Omim: 154030 Human
      • SwissProt: P67809 Human
      • SwissProt: P62960 Mouse
      • SwissProt: P62961 Rat
      • Unigene: 473583 Human
      • Unigene: 258204 Mouse
      • Unigene: 110976 Rat
      • Unigene: 194909 Rat
      see all
    • Alternative names

      • BP 8 antibody
      • CBF-A antibody
      • CCAAT binding transcription factor I subunit A antibody
      • CCAAT-binding transcription factor I subunit A antibody
      • CSDA2 antibody
      • CSDB antibody
      • DBPB antibody
      • DNA binding protein B antibody
      • DNA-binding protein B antibody
      • EFI-A antibody
      • Enhancer factor I subunit A antibody
      • MDR NF1 antibody
      • MGC104858 antibody
      • MGC110976 antibody
      • MGC117250 antibody
      • NSEP 1 antibody
      • NSEP1 antibody
      • Nuclease sensitive element binding protein 1 antibody
      • Nuclease-sensitive element-binding protein 1 antibody
      • p50 antibody
      • Q15905 antibody
      • Y-box binding protein 1 antibody
      • Y-box transcription factor antibody
      • Y-box-binding protein 1 antibody
      • YB 1 antibody
      • YB-1 antibody
      • YBOX1_HUMAN antibody
      • YBX 1 antibody
      • ybx1 antibody
      see all

    Images

    • Western blot - Anti-YB1 (phospho S102) antibody (ab138654)
      Western blot - Anti-YB1 (phospho S102) antibody (ab138654)
      Anti-YB1 (phospho S102) antibody (ab138654) at 1/500 dilution + HepG2 cell extract (from cells treated with PMA at 125ng/ml for 15 minutes) at 30 µg

      Predicted band size: 36 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab138654? Please let us know so that we can cite the reference in this datasheet.

    ab138654 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    We have AB74162 on order and the release date is for early January. Also it looks like this product has been on backorder for some time due to production issues. Can you please let me know if you offer an alternative antibody for this product that I can suggest to my end-user.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2012

    Answer

    Thank you for contacting us.

    The only alternative antibody to YB1 (phospho S102) is ab138654 which has been tested in WB and ELISA and with human samples. It is predicted to react with mouse and rat, but not guaranteed.
    https://www.abcam.com/yb1-phospho-s102-antibody-ab138654.html


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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