Product nameYeast nuclei isolation kit
Abcam’s Yeast nuclei isolation kit (ab206997) provides an easy and fast method for isolation of nuclei from yeast cells for downstream applications such as DNA-protein interaction, RNA-protein interaction and protein-protein interaction studies, DNase I foot-printing analysis, enzymatic assays, pull-down assays, as well as western blot and ELISA.
To understand the nature of the replication and expression of the yeast genome, it is essential to have a method for the preparation of nuclei at different stages of growth. Abcam’s Yeast nuclei isolation kit enables easy and fast purification of nuclei from yeast cells using yeast cell wall lysis and homogenization.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 50 tests 1 M DTT 1 x 1ml Buffer A 1 x 50ml Buffer B 1 x 50ml Buffer C 1 x 90ml Lysis Enzyme Cocktail 1 x 500µl Protease Inhibitor Cocktail (Lyophilized) (22mg) 1 vial
Functional Activity of Isolated Yeast Nuclei. Purified yeast nuclei were analyzed for HAT enzyme activity using H3 substrate. In yeast, H3-dependent HAT activity is nuclear specific. Positive Control: Nuclear Extract (NE, 4 mg/mL). Purified yeast nuclei show significant HAT Enzyme Activity. Yeast nuclei were isolated following the kit protocol.
ab206997 has not yet been referenced specifically in any publications.