Recombinant Anti-Yes1 antibody [EPR3172] (ab133314)
![Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)](https://www.abcam.com/ps/products/133/ab133314/Images/ab133314-146807-anti-yes1-antibody-epr3172-western-blot.jpg "Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3172] to Yes1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Yes1 antibody [EPR3172]
See all Yes1 primary antibodies -
Description
Rabbit monoclonal [EPR3172] to Yes1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Yes1 aa 1-100 (internal sequence). The exact sequence is proprietary.
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Positive control
- U87-MG, Molt-4, LnCaP and A431 cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3172 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
Target
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Function
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
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Database links
- Entrez Gene: 7525 Human
- Omim: 164880 Human
- SwissProt: P07947 Human
- Unigene: 194148 Human
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Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
Images
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Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)All lanes : Anti-Yes1 antibody [EPR3172] (ab133314) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
![Anti-Yes1 antibody [EPR3172] (ab133314)](https://www.abcam.com/ps/products/133/ab133314/Images/ab133314-2-benefits-of-recombinant-antibodies.png)
Anti-Yes1 antibody [EPR3172] (ab133314)
Protocols
Datasheets and documents
References (1)
ab133314 has been referenced in 1 publication.
- Goya Grocin A et al. Whole Proteome Profiling of N-Myristoyltransferase Activity and Inhibition Using Sortase A. Mol Cell Proteomics 18:115-126 (2019). PubMed: 30341083
