Recombinant Anti-Yes1 antibody [EPR3172] (ab133314)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Yes1 antibody [EPR3172]
See all Yes1 primary antibodies -
Description
Rabbit monoclonal [EPR3172] to Yes1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Yes1 aa 1-100 (internal sequence). The exact sequence is proprietary.
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Positive control
- U87-MG, Molt-4, LnCaP and A431 cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3172 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 60 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
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Database links
- Entrez Gene: 7525 Human
- Omim: 164880 Human
- SwissProt: P07947 Human
- Unigene: 194148 Human
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Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
Images
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![Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)](https://www.abcam.com/ps/products/133/ab133314/Images/ab133314-146807-anti-yes1-antibody-epr3172-western-blot.jpg)
Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)All lanes : Anti-Yes1 antibody [EPR3172] (ab133314) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Datasheets and documents
References
This product has been referenced in:
- Goya Grocin A et al. Whole Proteome Profiling of N-Myristoyltransferase Activity and Inhibition Using Sortase A. Mol Cell Proteomics 18:115-126 (2019). Read more (PubMed: 30341083) »
