Product nameAnti-Yes1 antibody [EPR3173]
See all Yes1 primary antibodies
DescriptionRabbit monoclonal [EPR3173] to Yes1
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Yes1 aa 500-600. The exact sequence is proprietary.
- WB: U-87MG, SW480 and Jurkat cell lysates IHC-P: Human colon tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionPromotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Cellular localizationCytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies.
- Information by UniProt
FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
ab109265 at 1/50 dilution staining Yes1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-Yes1 antibody [EPR3173] (ab109265) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Zhou W et al. Effect of miR-140-5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism. Exp Ther Med 18:1350-1356 (2019). Read more (PubMed: 31363375) »
- Liu L et al. Long noncoding RNA H19 competitively binds miR-17-5p to regulate YES1 expression in thyroid cancer. FEBS J 283:2326-39 (2016). Read more (PubMed: 27093644) »