Overview

  • Product name

    Anti-Yes1 antibody [EPR3173]
    See all Yes1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3173] to Yes1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Yes1 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • WB: U-87MG, SW480 and Jurkat cell lysates IHC-P: Human colon tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Autophosphorylated.
    • Cellular localization

      Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies.
    • Information by UniProt
    • Database links

    • Form

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • Alternative names

      • c Yes antibody
      • Cellular Yes 1 protein antibody
      • Cellular Yes1 protein antibody
      • HsT441 antibody
      • P61 YES antibody
      • p61-Yes antibody
      • Proto oncogene tyrosine protein kinase YES antibody
      • Proto-oncogene c-Yes antibody
      • Tyrosine-protein kinase Yes antibody
      • v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody
      • Viral oncogene yes 1 homolog 1 antibody
      • Viral oncogene yes1 homolog 1 antibody
      • Yamaguchi sarcoma oncogene antibody
      • Yes 1 antibody
      • Yes antibody
      • YES proto oncogene 1, Src family tyrosine kinase antibody
      • YES_HUMAN antibody
      • YES1 antibody
      • YES1 proto oncogene, Src family tyrosine kinase antibody
      see all

    Images

    • ab109265 at 1/50 dilution staining Yes1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-Yes1 antibody [EPR3173] (ab109265) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 61 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Zhou W  et al. Effect of miR-140-5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism. Exp Ther Med 18:1350-1356 (2019). Read more (PubMed: 31363375) »
    • Liu L  et al. Long noncoding RNA H19 competitively binds miR-17-5p to regulate YES1 expression in thyroid cancer. FEBS J 283:2326-39 (2016). Read more (PubMed: 27093644) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer


    I have included the antigen retrieval protocol below:

    Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0

    Antigen Retrieval

    Immerse slides into staining dish containing Antigen Retrieval Solution.

    Place covered staining dish into the rice cooker. Add 120 mL dH2O and press “cook”.

    When “cook” is turned to “warm” (about 20–30 min), unplug the cooker and remove the staining dish to the bench top.

    Allow to cool down, without cover, for 20 min.

    1) What is the exact problem you are encountering?
    2) What samples/species are you using?
    3) Which antibody dilution/incubation time are you using?

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up