Key features and details
- Rabbit polyclonal to YIF1A
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-YIF1A antibody
DescriptionRabbit polyclonal to YIF1A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
- WB: RT-4 and U-251 MG cell lysates. IHC-P: Human prostate, colon, skeletal muscle and pancreas tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionPossible role in transport between endoplasmic reticulum and Golgi.
Sequence similaritiesBelongs to the YIF1 family.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the endoplasmic reticulum-Golgi intermediate compartment.
- Information by UniProt
- 54TM antibody
- 54TMp antibody
- FinGER7 antibody
All lanes : Anti-YIF1A antibody (ab121455) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Immunohistochemical analysis of human prostate tissue lableing YIF1A with ab121455 at 1/200 dilution.
Immunohistochemical analysis of human colon tissue lableing YIF1A with ab121455 at 1/200 dilution.
Immunohistochemical analysis of human skeletal muscle tissue lableing YIF1A with ab121455 at 1/200 dilution.
Immunohistochemical analysis of human pancreas tissue lableing YIF1A with ab121455 at 1/200 dilution.
ab121455 has not yet been referenced specifically in any publications.