Key features and details
- Rabbit polyclonal to YIPF3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-YIPF3 antibody
See all YIPF3 primary antibodies
DescriptionRabbit polyclonal to YIPF3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- WB: Mouse heart, liver, brain and stomach lysates. IHC-P: Human colon cancer tissue. ICC/IF: PC-3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionMay play a role in hematopoiesis.
Tissue specificityExpressed by nucleated hematopoietic cells (at protein level).
Sequence similaritiesBelongs to the YIP1 family.
Developmental stageExpressed in fetal liver (at protein level). Expressed in embryonic hematopoiesis sites.
Cellular localizationCell membrane. Cytoplasm. Localization to the cytoplasm or to the cell membrane is developmentally and ontogenetically regulated.
- Information by UniProt
- C6orf109 antibody
- dJ337H4.3 antibody
- FinGER3 antibody
All lanes : Anti-YIPF3 antibody (ab223505) at 1/500 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse stomach lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Paraffin-embedded human colon cancer tissue stained for YIPF3 using ab223505 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for YIPF3 (green) using ab223505 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
ab223505 has not yet been referenced specifically in any publications.