Key features and details
- Rabbit polyclonal to YKL-40/CHI3L1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-YKL-40/CHI3L1 antibody
See all YKL-40/CHI3L1 primary antibodies
DescriptionRabbit polyclonal to YKL-40/CHI3L1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig, Orangutan
Recombinant full length protein within Human YKL-40/CHI3L1 aa 22-240. The exact sequence is proprietary.
MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFL CTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWN FGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHF TTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDF ISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAP ASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEIC DFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAM VWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Database link: P36222
- WB: MCF-7 and HT-29 cell lysates; IHC: Mouse spleen and liver tissues; ICC/ IF: Raw264.7 cells.
Previously labelled as CHI3L1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180569 in the following tested applications.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCarbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
Tissue specificityPresent in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Involvement in diseaseA genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
Sequence similaritiesBelongs to the glycosyl hydrolase 18 family.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- 39 kDa synovial protein antibody
- ASRT7 antibody
- Cartilage glycoprotein 39 antibody
Paraffin-embedded mouse spleen tissue stained for YKL-40 using ab180569 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of Raw264.7 cells stained for YKL-40 with ab180569 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-YKL-40/CHI3L1 antibody (ab180569) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HT-29 cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat AntiRabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 30 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Paraffin-embedded mouse liver tissue stained for YKL-40 using ab180569 at 1/100 dilution in immunohistochemical analysis.
ab180569 has been referenced in 1 publication.
- Chen C et al. FOXO1 associated with sensitivity to chemotherapy drugs and glial-mesenchymal transition in glioma. J Cell Biochem 120:882-893 (2019). PubMed: 30216501