Overview

  • Product name

    Anti-YKL-40/CHI3L1 antibody
    See all YKL-40/CHI3L1 primary antibodies
  • Description

    Rabbit polyclonal to YKL-40/CHI3L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human YKL-40/CHI3L1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88218)

  • Positive control

    • This antibody gave a positive signal in THP1 Whole Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.
  • General notes

    Previously labelled as CHI3L1

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
  • Tissue specificity

    Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
  • Involvement in disease

    A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 18 family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • 39 kDa synovial protein antibody
    • ASRT7 antibody
    • Cartilage glycoprotein 39 antibody
    • CGP-39 antibody
    • CGP39 antibody
    • CH3L1_HUMAN antibody
    • CHI3L1 antibody
    • chitinase 3 like 1 (cartilage glycoprotein 39) antibody
    • chitinase 3 like 1 antibody
    • Chitinase 3 like protein 1 precursor antibody
    • chitinase antibody
    • Chitinase-3-like protein 1 antibody
    • Chondrocyte protein YKL40 antibody
    • GP 39 antibody
    • GP-39 antibody
    • GP39 antibody
    • HC gp39 antibody
    • HCGP 3P antibody
    • hCGP-39 antibody
    • HCgp39 antibody
    • YKL 40 antibody
    • YKL-40 antibody
    • YKL40 antibody
    • YYL 40 antibody
    see all

Images

  • Anti-YKL-40/CHI3L1 antibody (ab77528) at 1 µg/ml + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

  • IHC image of YKL-40/CHI3L1 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab77528 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77528 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Pouyafar A  et al. Treatment of cancer stem cells from human colon adenocarcinoma cell line HT-29 with resveratrol and sulindac induced mesenchymal-endothelial transition rate. Cell Tissue Res N/A:N/A (2019). Read more (PubMed: 30758710) »
  • Qiu QC  et al. CHI3L1 promotes tumor progression by activating TGF-ß signaling pathway in hepatocellular carcinoma. Sci Rep 8:15029 (2018). Read more (PubMed: 30301907) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
35 µg
Treatment
5% ethanol for 10 days
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Ms. Ji Hye Han

Verified customer

Submitted Oct 23 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up