RecombinantRabMAb

Recombinant Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
  • Flow Cytometry - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
  • Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19078-157] to YKL-40/CHI3L1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-YKL-40/CHI3L1 antibody [EPR19078-157]
    See all YKL-40/CHI3L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19078-157] to YKL-40/CHI3L1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human YKL-40/CHI3L1 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P36222

  • Positive control

    • WB: Human spleen tissue lysate; THP-1 (Human monocytic leukemia monocyte), U-87 MG ( Human glioblastoma-astrocytoma epithelial cell), MG-63 (Human osteosarcoma fibroblast), Caco-2 (Human colorectal adenocarcinoma epithelial cell), 293T (Human embryonic kidney epithelial cell), and Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates; IHC-P: Human spleen and tonsil tissue. Flow: THP-1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19078-157

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab255297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.

    • Tissue specificity

      Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

    • Involvement in disease

      A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.

    • Sequence similarities

      Belongs to the glycosyl hydrolase 18 family.

    • Post-translational
      modifications

      Glycosylated.

    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt

    • Database links

      • Alternative names

        • 39 kDa synovial protein antibody
        • ASRT7 antibody
        • Cartilage glycoprotein 39 antibody
        • CGP-39 antibody
        • CGP39 antibody
        • CH3L1_HUMAN antibody
        • CHI3L1 antibody
        • chitinase 3 like 1 (cartilage glycoprotein 39) antibody
        • chitinase 3 like 1 antibody
        • Chitinase 3 like protein 1 precursor antibody
        • chitinase antibody
        • Chitinase-3-like protein 1 antibody
        • Chondrocyte protein YKL40 antibody
        • GP 39 antibody
        • GP-39 antibody
        • GP39 antibody
        • HC gp39 antibody
        • HCGP 3P antibody
        • hCGP-39 antibody
        • HCgp39 antibody
        • YKL 40 antibody
        • YKL-40 antibody
        • YKL40 antibody
        • YYL 40 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

        Immunohistochemical analysis of paraffin-embedded human spleen  tissue labeling YKL-40/CHI3L1 with ab255297 at 1/250 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils of human spleen is observed.

        The section was incubated with ab255297 for 30 mins at room temperature.

        The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20mins.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      • Flow Cytometry - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        Flow Cytometry - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

        Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized THP-1 (human monocytic leukemia cell line) cells labelling YKL-40/CHI3L1 with ab255297 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

        Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        All lanes : Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297) at 1/1000 dilution

        Lane 1 : Human spleen tissue lysate
        Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
        Lane 3 : U-87 MG ( Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
        Lane 4 : MG-63 (Human osteosarcoma fibroblast) whole cell lysate
        Lane 5 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
        Lane 6 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
        Lane 7 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 43 kDa
        Observed band size: 42 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 60 seconds


        Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

        The expression profile observed in 293T and Jurkat are consistent with the literature (PMID: 19767768,PMID: 16472595).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

        Immunohistochemical analysis of paraffin-embedded human tonsil  tissue labeling YKL-40/CHI3L1 with ab255297 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in human tonsil germinal center is observed. The section was incubated with ab255297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        All lanes : Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297) at 1/1000 dilution

        Lane 1 : Human spleen lysate
        Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
        Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 43 kDa
        Observed band size: 42 kDa why is the actual band size different from the predicted?



        Blocking/Diluting buffer and concentration: 5% NFDM/TBST

        Exposure time: 3 minutes

        The molecular weight observed is consistent with what has been described in the literature (PMID 19414556).

      • Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)
        Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab255297? Please let us know so that we can cite the reference in this datasheet.

      ab255297 has not yet been referenced specifically in any publications.

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