Recombinant Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-1-anti-ykl40chi3l1-antibody-epr23891162-immunohistochemistry-mouse-lung.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23891-162] to YKL-40/CHI3L1
- Suitable for: IHC-P, WB
- Reacts with: Mouse
Overview
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Product name
Anti-YKL-40/CHI3L1 antibody [EPR23891-162]
See all YKL-40/CHI3L1 primary antibodies -
Description
Rabbit monoclonal [EPR23891-162] to YKL-40/CHI3L1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: IHC-Fr or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse YKL-40/CHI3L1 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q61362 -
Positive control
- WB: Mouse spleen and lung tissue lysate. IHC-P: Mouse lung, spleen and colon cancer tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23891-162 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab259322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 43 kDa. |
Target
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Function
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. -
Tissue specificity
Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. -
Involvement in disease
A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 12654 Mouse
- SwissProt: Q61362 Mouse
- Unigene: 38274 Mouse
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Alternative names
- 39 kDa synovial protein antibody
- ASRT7 antibody
- Cartilage glycoprotein 39 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse lung tissues (PMID:26528713). The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Western blot - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-448946-anti-ykl40chi3l1-antibody-epr23891162-western-blot-lysate.jpg)
Western blot - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)All lanes : Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDa
Observed band size: 43 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 81 seconds
Lane 2: 10 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-2-anti-ykl40chi3l1-antibody-epr23891162-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse spleen tissue. The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-3-anti-ykl40chi3l1-antibody-epr23891162-immunohistochemistry-mouse-colon-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)Immunohistochemical analysis of paraffin-embedded Mouse colon cancer tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on immune cells of mouse colon cancer tissues. The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab259322 has not yet been referenced specifically in any publications.
