Anti-YL1 antibody (ab112055)
Key features and details
- Rabbit polyclonal to YL1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YL1 antibody
See all YL1 primary antibodies -
Description
Rabbit polyclonal to YL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 50-100 of Human YL1 (NP_005988.1)
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Positive control
- Whole cell lysate from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 41 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling. -
Sequence similarities
Belongs to the VPS72/YL1 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 534883 Cow
- Entrez Gene: 6944 Human
- Entrez Gene: 21427 Mouse
- Entrez Gene: 100173671 Orangutan
- Entrez Gene: 310661 Rat
- Omim: 600607 Human
- SwissProt: Q5E9F6 Cow
- SwissProt: Q15906 Human
see all -
Alternative names
- CFL1 antibody
- HGNC11644 antibody
- Protein YL-1 antibody
see all
Images
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Immunoprecipitation of YL1. Samples: 1mg of whole cell lysate from HeLa cells(20% of IP loaded). Antibodies: Affinity purified rabbit anti-YL1 antibody (ab112055) was used at 1 µg/ml. Lane 1- YL1 was also immunoprecipitated by rabbit anti-YL1 antibody (ab112055) at 6 µg/mg lysate. Lane 2- YL1 was immunoprecipitated by an rabbit anti-YL1 antibody that recognizes a downstream epitope. 3- Rabbit IgG control.Detection: Chemiluminescence with exposure time of 10 seconds.
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All lanes : Anti-YL1 antibody (ab112055) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 41 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab112055 has been referenced in 1 publication.
- Zhao B et al. Znhit1 controls intestinal stem cell maintenance by regulating H2A.Z incorporation. Nat Commun 10:1071 (2019). PubMed: 30842416