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    yl1-antibody-ab167639.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-YL1 antibody (ab167639)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-YL1 antibody (ab167639)
  • Western blot - Anti-YL1 antibody (ab167639)

Key features and details

  • Mouse polyclonal to YL1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-YL1 antibody
    See all YL1 primary antibodies
  • Description

    Mouse polyclonal to YL1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-364 of Human YL1 (NP_005988.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • YL1 transfected 293T cell line; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)

Applications

Our Abpromise guarantee covers the use of ab167639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.
  • Sequence similarities

    Belongs to the VPS72/YL1 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15906 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 534883 Cow
    • Entrez Gene: 6944 Human
    • Entrez Gene: 21427 Mouse
    • Entrez Gene: 100173671 Orangutan
    • Omim: 600607 Human
    • SwissProt: Q5E9F6 Cow
    • SwissProt: Q15906 Human
    • SwissProt: Q62481 Mouse
    • SwissProt: Q5R5V9 Orangutan
    • Unigene: 2430 Human
    • Unigene: 133919 Mouse
    see all
  • Alternative names

    • CFL1 antibody
    • HGNC11644 antibody
    • Protein YL-1 antibody
    • Swc2 antibody
    • TCFL1 antibody
    • Transcription factor like 1 (YL1) antibody
    • Transcription Factor Like 1 antibody
    • Transcription factor-like 1 antibody
    • Vacuolar protein sorting 72 (yeast) antibody
    • Vacuolar protein sorting 72 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 72 homolog antibody
    • Vacuolar protein sorting-associated protein 72 homolog antibody
    • VPS72 antibody
    • VPS72_HUMAN antibody
    • YL-1 antibody
    • YL1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-YL1 antibody (ab167639)
    Immunocytochemistry/ Immunofluorescence - Anti-YL1 antibody (ab167639)
    Immunofluorescence analysis of HeLa cells, labeling YL1 with ab167639 10 µg/ml
  • Western blot - Anti-YL1 antibody (ab167639)
    Western blot - Anti-YL1 antibody (ab167639)
    All lanes : Anti-YL1 antibody (ab167639) at 1 µg/ml

    Lane 1 : YL1 transfected 293T cell line
    Lane 2 : Non-transfected 293T cell line

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 41 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab167639? Please let us know so that we can cite the reference in this datasheet.

    ab167639 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-YL1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Kidney cell line)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    3000 cells
    Specification
    Kidney cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2016

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