Application
Western blot
Sample
Human Cell lysate - nuclear (Kidney cell line)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
3000 cells
Specification
Kidney cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Concentration
1 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
293T transfected with GFP as a transfection control.
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 41, 65 kDa
Positive control
293T transfected with hemagglutinin (HA) tagged YL-1: overexpressed YL-1 line.
Additional data
Additional Notes
Lane 1 - 293T (human embryonic kidney cell line) with transfection control (GFP inserted into NSBI vector). Lane 2 - 293T with hemagglutinin (HA) tagged YL-1 . Bands at 41 and 65 kDa likely specific to YL-1. Better visualization is likely with higher antibody concentration.
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Submitted Nov 30 2016