Overview

  • Product name

    Anti-Ym-1 + Ym-2 antibody [EPR15263]
    See all Ym-1 + Ym-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse Ym-1 + Ym-2 aa 200-350. The exact sequence is proprietary.
    Database link: O35744

  • Positive control

    • WB: Mouse lung, mouse brain, mouse heart, C6, Raw264.7, PC-12 and NIH3T3 lysates. IHC-P: Mouse liver tissue. ICC/IF: Raw264.7 cells Flow Cyt: C2C12 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15263
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 45 kDa (predicted molecular weight: 44, 45 kDa).
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200.

Target

  • Relevance

    Chitinase 3 like protein 3: Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy. Chitinase 3 like protein 4: Has low chemotactic activity for eosinophils. May play a role in inflammation and allergy. Has no chitinase activity.
  • Cellular localization

    Chitinase 3 like protein 3: Secreted. Rough endoplasmic reticulum lumen. Nucleus envelope. Cytoplasm. Cytoplasmic granule. Note: Predominantly localizes to the lumen of rough endoplasmic reticulum (rER) and nuclear envelope in alveolar macrophages. Localizes to the dilated lumen of rER in immature neutrophils in spleen and in cytoplasmic granules in peritoneal neutrophils. Detected in needle-shaped crystals present in the cytoplasm of bone marrow macrophages. Chitinase 3 like protein 4: Secreted. Cytoplasm. Note: Detected in the cytoplasm of keratinocytes.
  • Database links

  • Alternative names

    • Chi3l3 antibody
    • Beta-N-acetylhexosaminidase Ym1 antibody
    • Chi3l4 antibody
    • Chil3 antibody
    • Chil4 antibody
    • Chitinase-3-like protein 3 antibody
    • Chitinase-3-like protein 4 antibody
    • Chitinase-like protein 3 antibody
    • Chitinase-like protein 4 antibody
    • ECF-L antibody
    • Eosinophil chemotactic cytokine antibody
    • Secreted protein Ym1 antibody
    • Secreted protein Ym2 antibody
    • Ym1 antibody
    • Ym2 antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed Raw264.7 cells labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/200, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 (ab150077) and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution; 2. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/500 with prediluted HRP polymer for Rabbit IgG as secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometrical analysis of paraformaldehyde-fixed C2C12 cells labeling Chitinase 3 like protein 3 + Chitinase 3 with ab192029 at 1/200 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • All lanes : Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : C6 lysate
    Lane 4 : Raw264.7 lysate
    Lane 5 : PC-12 lysate
    Lane 6 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44, 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/50000 dilution + Mouse lung lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44, 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Cavalcante PAM  et al. Nephropathy in Hypertensive Animals Is Linked to M2 Macrophages and Increased Expression of the YM1/Chi3l3 Protein. Mediators Inflamm 2019:9086758 (2019). Read more (PubMed: 31360120) »
  • Camolotto SA  et al. FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer. Elife 7:N/A (2018). Read more (PubMed: 30475207) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ^Target Retrival Low ph
Permeabilization
No
Specification
Liver
Blocking step
Peroxidase Block as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Frau. Ines Kleiber-Schaaf

Verified customer

Submitted Apr 30 2018

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