Overview

  • Product name

    Anti-Ym-1 + Ym-2 antibody [EPR15263] (Alexa Fluor® 488)
    See all Ym-1 + Ym-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Mouse Chitinase 3 like protein 3 + Chitinase 3 like protein 4 aa 200-350. The exact sequence is proprietary.
    Database link: O35744

  • Positive control

    • ICC/IF: RAW264.7 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15263
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min)

Target

  • Relevance

    Chitinase 3 like protein 3: Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy. Chitinase 3 like protein 4: Has low chemotactic activity for eosinophils. May play a role in inflammation and allergy. Has no chitinase activity.
  • Cellular localization

    Chitinase 3 like protein 3: Secreted. Rough endoplasmic reticulum lumen. Nucleus envelope. Cytoplasm. Cytoplasmic granule. Note: Predominantly localizes to the lumen of rough endoplasmic reticulum (rER) and nuclear envelope in alveolar macrophages. Localizes to the dilated lumen of rER in immature neutrophils in spleen and in cytoplasmic granules in peritoneal neutrophils. Detected in needle-shaped crystals present in the cytoplasm of bone marrow macrophages. Chitinase 3 like protein 4: Secreted. Cytoplasm. Note: Detected in the cytoplasm of keratinocytes.
  • Database links

  • Alternative names

    • Chi3l3 antibody
    • Beta-N-acetylhexosaminidase Ym1 antibody
    • Chi3l4 antibody
    • Chil3 antibody
    • Chil4 antibody
    • Chitinase-3-like protein 3 antibody
    • Chitinase-3-like protein 4 antibody
    • Chitinase-like protein 3 antibody
    • Chitinase-like protein 4 antibody
    • ECF-L antibody
    • Eosinophil chemotactic cytokine antibody
    • Secreted protein Ym1 antibody
    • Secreted protein Ym2 antibody
    • Ym1 antibody
    • Ym2 antibody
    see all

Images

  • ab205490 staining Chitinase 3 like protein 3 + Chitinase 3 like protein 4 in RAW246.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205490 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab205490 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205490.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up