Overview

  • Product name

    Anti-Ym-1 + Ym-2 antibody [EPR15263] (Phycoerythrin)
    See all Ym-1 + Ym-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Mouse Chitinase 3 like protein 3 + Chitinase 3 like protein 4 aa 200-350. The exact sequence is proprietary.
    Database link: O35744

  • Positive control

    • Flow Cytometry: RAW264.7 cells ICC/IF: RAW264.7 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15263
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min), and 4% formaldehyde.

Flow Cyt 1/1000.

Target

  • Relevance

    Chitinase 3 like protein 3: Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy. Chitinase 3 like protein 4: Has low chemotactic activity for eosinophils. May play a role in inflammation and allergy. Has no chitinase activity.
  • Cellular localization

    Chitinase 3 like protein 3: Secreted. Rough endoplasmic reticulum lumen. Nucleus envelope. Cytoplasm. Cytoplasmic granule. Note: Predominantly localizes to the lumen of rough endoplasmic reticulum (rER) and nuclear envelope in alveolar macrophages. Localizes to the dilated lumen of rER in immature neutrophils in spleen and in cytoplasmic granules in peritoneal neutrophils. Detected in needle-shaped crystals present in the cytoplasm of bone marrow macrophages. Chitinase 3 like protein 4: Secreted. Cytoplasm. Note: Detected in the cytoplasm of keratinocytes.
  • Database links

  • Alternative names

    • Chi3l3 antibody
    • Beta-N-acetylhexosaminidase Ym1 antibody
    • Chi3l4 antibody
    • Chil3 antibody
    • Chil4 antibody
    • Chitinase-3-like protein 3 antibody
    • Chitinase-3-like protein 4 antibody
    • Chitinase-like protein 3 antibody
    • Chitinase-like protein 4 antibody
    • ECF-L antibody
    • Eosinophil chemotactic cytokine antibody
    • Secreted protein Ym1 antibody
    • Secreted protein Ym2 antibody
    • Ym1 antibody
    • Ym2 antibody
    see all

Images

  • ab211621 staining Chitinase 3 like protein 3 + Chitinase 3 like protein 4  in RAW264.7 cells. The cells were fixed with 100% methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211621 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue). 

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

    This product also gave a positive signal under the same testing conditions in RAW264.7 cells fixed with 4% formaldehyde.

  • Overlay histogram showing RAW264.7 cells stained with ab211621 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211621, 1/1000 dilution) for 30 min at 22°C.        

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 

    Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.               

    This antibody gave a positive signal in RAW264.7 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab211621 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211621.
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