Product nameAnti-YME1L1 antibody
See all YME1L1 primary antibodies
DescriptionRabbit polyclonal to YME1L1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human YME1L1 aa 395-600.
MHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQ VTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVN QAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHES GHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQM DVSMGG
Database link: BC023507
- HeLa, HepG2, 7721, U373, MCF7 cell lysates; Human colon cancer tissue.
Previously labelled as ATP-dependent metalloprotease YME1L1.
FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab170123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/200 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).|
FunctionPutative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1.
Tissue specificityHigh expression in cardiac and skeletal muscle mitochondria.
Sequence similaritiesIn the N-terminal section; belongs to the AAA ATPase family.
In the C-terminal section; belongs to the peptidase M41 family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- ATP dependent metalloprotease FtsH1 homolog antibody
- ATP-dependent metalloprotease FtsH1 antibody
- ATP-dependent metalloprotease YME1L1 antibody
All lanes : Anti-YME1L1 antibody (ab170123) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 7721 cell lysate
Lane 4 : U373 cell lysate
Lane 5 : MCF7 cell lysate
Predicted band size: 86 kDa
Observed band size: 86 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon cancer tissue labeling YME1L1 with ab170123 at 1/100 dilution.
This product has been referenced in:
- Ogunbileje JO et al. Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma. Am J Physiol Endocrinol Metab 311:E436-48 (2016). Read more (PubMed: 27382037) »
- Akman HB et al. 3'UTR shortening and EGF signaling: implications for breast cancer. Hum Mol Genet 24:6910-20 (2015). Read more (PubMed: 26395459) »