Anti-YME1L1 antibody (ab234744)
Key features and details
- Rabbit polyclonal to YME1L1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YME1L1 antibody
See all YME1L1 primary antibodies -
Description
Rabbit polyclonal to YME1L1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human YME1L1 aa 1-240.
Database link: Q96TA2 -
Positive control
- WB: A549, jurkat, A431, HeLa, HepG2 and MCF7 cell lysates. IHC-P: Human tonsil and colon cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa). |
Target
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Function
Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1. -
Tissue specificity
High expression in cardiac and skeletal muscle mitochondria. -
Sequence similarities
In the N-terminal section; belongs to the AAA ATPase family.
In the C-terminal section; belongs to the peptidase M41 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 10730 Human
- Omim: 607472 Human
- SwissProt: Q96TA2 Human
- Unigene: 499145 Human
- Unigene: 74647 Human
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Alternative names
- ATP dependent metalloprotease FtsH1 homolog antibody
- ATP-dependent metalloprotease FtsH1 antibody
- ATP-dependent metalloprotease YME1L1 antibody
see all
Images
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All lanes : Anti-YME1L1 antibody (ab234744) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human tonsil tissue stained for YME1L1 using ab234744 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon cancer tissue stained for YME1L1 using ab234744 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab234744 has been referenced in 1 publication.
- Pang Y et al. HIGD-1B inhibits hypoxia-induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24:N/A (2021). PubMed: 34080026