Key features and details
- Rabbit polyclonal to YME1L1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-YME1L1 antibody
See all YME1L1 primary antibodies
DescriptionRabbit polyclonal to YME1L1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human YME1L1 aa 1-240.
Database link: Q96TA2
- WB: A549, jurkat, A431, HeLa, HepG2 and MCF7 cell lysates. IHC-P: Human tonsil and colon cancer tissues.
Previously labelled as ATP-dependent metalloprotease YME1L1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
Our Abpromise guarantee covers the use of ab234744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa).|
FunctionPutative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1.
Tissue specificityHigh expression in cardiac and skeletal muscle mitochondria.
Sequence similaritiesIn the N-terminal section; belongs to the AAA ATPase family.
In the C-terminal section; belongs to the peptidase M41 family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- ATP dependent metalloprotease FtsH1 homolog antibody
- ATP-dependent metalloprotease FtsH1 antibody
- ATP-dependent metalloprotease YME1L1 antibody
All lanes : Anti-YME1L1 antibody (ab234744) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Paraffin-embedded human tonsil tissue stained for YME1L1 using ab234744 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for YME1L1 using ab234744 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234744 has not yet been referenced specifically in any publications.