Key features and details
- Rabbit polyclonal to YTHDC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-YTHDC1 antibody
See all YTHDC1 primary antibodies
DescriptionRabbit polyclonal to YTHDC1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human esophagus, fallopian tube, duodenum, endometrium and testis tissues. WB: RT-4 and U-251 MG cell lysates. ICC/IF: HeLa and U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500. Predicted molecular weight: 85 kDa.|
FunctionMay be part of a signal transduction pathway that influences splice site selection.
Sequence similaritiesContains 1 YTH domain.
Cellular localizationNucleus. Localizes to a novel subnuclear structure, the YT bodies.
- Information by UniProt
- KIAA1966 antibody
- Putative splicing factor YT521 antibody
- splicing factor YT521-B antibody
All lanes : Anti-YTHDC1 antibody (ab122340) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 85 kDa
Paraffin-embedded human testis tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human duodenum tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for YTHDC1 (green) using ab122340 at 4 µg/ml in ICC/IF.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122340 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122340, at a 1/400 dilution, staining YTHDC1 in paraffin-embedded Human esophagus tissue by immunohistochemistry.
Immunohistochemical staining of Human fallopian tube shows strong nuclear positivity in glandular cells using ab122340
ab122340 has been referenced in 3 publications.
- Chen RX et al. N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis. Nat Commun 10:4695 (2019). PubMed: 31619685
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244
- Patil DP et al. m(6)A RNA methylation promotes XIST-mediated transcriptional repression. Nature 537:369-373 (2016). PubMed: 27602518