Overview

  • Product name
    Anti-YTHDC2 antibody
  • Description
    Rabbit polyclonal to YTHDC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human YTHDC2 aa 1200-1250. The exact sequence is proprietary. NP_073739.3
    Sequence:

    K SSADTEFSDE CTTAERVLMK SPSPALHPPQ KYKDRGILHP KRGTEDRSDQ


    Database link: Q9H6S0

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176846 was affinity purified using an epitope specific to YTHDC2 immobilized on solid support
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 160 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • All lanes : Anti-YTHDC2 antibody (ab176846) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique.

    Predicted band size: 160 kDa


    Exposure time: 30 seconds
  • Detection of YTHDC2 by Western Blot of Immunoprecipitate.
    ab176846 at 0.4 µg/ml labeling YTHDC2 in HeLa whole cell lysate immunoprecipitated using ab176846 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:
  • Muller M & Glaunsinger BA Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases. PLoS Pathog 13:e1006593 (2017). WB . Read more (PubMed: 28841715) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human osteosarcoma U2OS cells)
Permeabilization
Yes - Triton X-100 0.2%
Specification
Human osteosarcoma U2OS cells
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 06 2015

Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (6% SDS-PAGE gel)
Sample
Human Cell lysate - whole cell (Human HCT116 cells)
Specification
Human HCT116 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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