Recombinant Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
![Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)](https://www.abcam.com/ps/products/245/ab245129/Images/ab245129-336275-anti-ythdf2-antibody-epr20318-western-blot.jpg "Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20318] to YTHDF2 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free
See all YTHDF2 primary antibodies -
Description
Rabbit monoclonal [EPR20318] to YTHDF2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mESC and HeLa whole cell lysate.
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General notes
ab245129 is the carrier-free version of ab220163.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20318 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa. |
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IP
Use at an assay dependent concentration. |
Target
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Sequence similarities
Contains 1 YTH domain. - Information by UniProt
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Database links
- Entrez Gene: 51441 Human
- Entrez Gene: 213541 Mouse
- Entrez Gene: 313053 Rat
- Omim: 610640 Human
- SwissProt: Q9Y5A9 Human
- SwissProt: Q91YT7 Mouse
- Unigene: 532286 Human
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Alternative names
- 9430020E02Rik antibody
- CAHL antibody
- CLL associated antigen KW 14 antibody
see all
Images
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Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution
Lane 1 : Wild-type mESC (mouse embryo stem cell) whole cell lysate
Lane 2 : YTHDF2 knockout mESC whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 62 kDa
Exposure time: 48 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220163).
The wild-type and YTHDF2 knockout cell lysates were kindly provided by an anonymous collaborator.
ab220163 was shown to specifically react with YTHDF2 in wild-type mESC cells as signal was lost in YTHDF2 knockout cells. Wild-type and YTHDF2 knockout samples were subjected to SDS-PAGE. ab220163 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
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![Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)](https://www.abcam.com/ps/products/245/ab245129/Images/ab245129-2-benefits-of-recombinant-antibodies.png)
Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab245129 has been referenced in 2 publications.
- Fu Y & Zhuang X m6A-binding YTHDF proteins promote stress granule formation. Nat Chem Biol 16:955-963 (2020). PubMed: 32451507
- Huang B et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). PubMed: 31316467
