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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
  • Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20318] to YTHDF2 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Product image
Recombinant Human YTHDF2 protein (ab153351)

View more associated products

Overview

  • Product name

    Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free
    See all YTHDF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20318] to YTHDF2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type mESC and HeLa whole cell lysate.
  • General notes

    Ab245129 is the carrier-free version of ab220163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245129 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20318
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Alternative Versions

    • Anti-YTHDF2 antibody [EPR20318] (ab220163)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Recombinant Protein

    • Recombinant Human YTHDF2 protein (ab153351)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab245129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Recombinant fragment
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IP
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Sequence similarities

      Contains 1 YTH domain.
    • Target information above from: UniProt accession Q9Y5A9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51441 Human
      • Entrez Gene: 213541 Mouse
      • Entrez Gene: 313053 Rat
      • Omim: 610640 Human
      • SwissProt: Q9Y5A9 Human
      • SwissProt: Q91YT7 Mouse
      • Unigene: 532286 Human
      • Alternative names

        • 9430020E02Rik antibody
        • CAHL antibody
        • CLL associated antigen KW 14 antibody
        • CLL-associated antigen KW-14 antibody
        • HGRG8 antibody
        • High glucose regulated protein 8 antibody
        • High-glucose-regulated protein 8 antibody
        • NY REN 2 antibody
        • Renal carcinoma antigen NY REN 2 antibody
        • Renal carcinoma antigen NY-REN-2 antibody
        • YTH domain family 2 antibody
        • YTH domain family member 2 antibody
        • YTH domain family protein 2 antibody
        • YTH N6 methyladenosine RNA binding protein 2 antibody
        • YTHD2_HUMAN antibody
        • YTHDF 2 antibody
        • YTHDF2 antibody
        see all

      Images

      • Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
        Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
        All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution

        Lane 1 : Wild-type mESC (mouse embryo stem cell) whole cell lysate
        Lane 2 : YTHDF2 knockout mESC whole cell lysate
        Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 62 kDa


        Exposure time: 48 seconds


        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220163).

        The wild-type and YTHDF2 knockout cell lysates were kindly provided by an anonymous collaborator.

        ab220163 was shown to specifically react with YTHDF2 in wild-type mESC cells as signal was lost in YTHDF2 knockout cells. Wild-type and YTHDF2 knockout samples were subjected to SDS-PAGE. ab220163 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

      • Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
        Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (1)

      Publishing research using ab245129? Please let us know so that we can cite the reference in this datasheet.

      ab245129 has been referenced in 1 publication.

      • Huang B  et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). PubMed: 31316467

      Customer reviews and Q&As

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