Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)

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Overview

  • Product name

    Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free
    See all YTHDF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20318] to YTHDF2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human YTHDF2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y5A9

  • Positive control

    • WB: Wild-type mESC and HeLa whole cell lysate.

  • General notes

    Ab245129 is the carrier-free version of ab220163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245129 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20318

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Sequence similarities

      Contains 1 YTH domain.
    • Information by UniProt

    • Database links

      • Alternative names

        • 9430020E02Rik antibody
        • CAHL antibody
        • CLL associated antigen KW 14 antibody
        • CLL-associated antigen KW-14 antibody
        • HGRG8 antibody
        • High glucose regulated protein 8 antibody
        • High-glucose-regulated protein 8 antibody
        • NY REN 2 antibody
        • Renal carcinoma antigen NY REN 2 antibody
        • Renal carcinoma antigen NY-REN-2 antibody
        • YTH domain family 2 antibody
        • YTH domain family member 2 antibody
        • YTH domain family protein 2 antibody
        • YTH N6 methyladenosine RNA binding protein 2 antibody
        • YTHD2_HUMAN antibody
        • YTHDF 2 antibody
        • YTHDF2 antibody
        see all

      Images

      • Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
        Western blot - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free (ab245129)
        All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution

        Lane 1 : Wild-type mESC (mouse embryo stem cell) whole cell lysate
        Lane 2 : YTHDF2 knockout mESC whole cell lysate
        Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 62 kDa


        Exposure time: 48 seconds


        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220163).

        The wild-type and YTHDF2 knockout cell lysates were kindly provided by an anonymous collaborator.

        ab220163 was shown to specifically react with YTHDF2 in wild-type mESC cells as signal was lost in YTHDF2 knockout cells. Wild-type and YTHDF2 knockout samples were subjected to SDS-PAGE. ab220163 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

      References

      This product has been referenced in:

      • Huang B  et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). Read more (PubMed: 31316467) »
      See 1 Publication for this product

      Publishing research using ab245129? Please let us know so that we can cite the reference in this datasheet.

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