Overview

  • Product name

  • Description

    Rabbit polyclonal to YTHDF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 235-265 of Human YTHDF3.

  • Positive control

    • A549 cell lysate; Human skeletal muscle tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab103328 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 64 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
  • Sequence similarities

    Contains 1 YTH domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ31657 antibody
    • YTH domain family 3 antibody
    • YTH domain family member 3 antibody
    • YTH domain family protein 3 antibody
    • YTH domain-containing family protein 3 antibody
    • YTHD3_HUMAN antibody
    • YTHDF 3 antibody
    • YTHDF3 antibody
    see all

Images

  • Anti-YTHDF3 antibody (ab103328) at 1/100 dilution + A549 cell lysate at 35 µg

    Predicted band size: 64 kDa

  • ab103328, at 1/50 dilution, staining YTHDF3 in formalin-fixed, paraffin-embedded Human skeletal muscle by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References

This product has been referenced in:

  • Lobo J  et al. m6A RNA modification and its writer/reader VIRMA/YTHDF3 in testicular germ cell tumors: a role in seminoma phenotype maintenance. J Transl Med 17:79 (2019). Read more (PubMed: 30866959) »
  • Panneerdoss S  et al. Cross-talk among writers, readers, and erasers of m6A regulates cancer growth and progression. Sci Adv 4:eaar8263 (2018). Read more (PubMed: 30306128) »
See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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