Product nameAnti-YTHDF3 antibody
See all YTHDF3 primary antibodies
DescriptionRabbit polyclonal to YTHDF3
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Hela cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83716 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
FunctionSpecifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
Sequence similaritiesContains 1 YTH domain.
- Information by UniProt
- FLJ31657 antibody
- YTH domain family 3 antibody
- YTH domain family member 3 antibody
Anti-YTHDF3 antibody (ab83716) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Huang B et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). Read more (PubMed: 31316467) »