Key features and details
- Rabbit polyclonal to YWHAE
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-YWHAE antibody
See all YWHAE primary antibodies
DescriptionRabbit polyclonal to YWHAE
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Chicken, Cow
Recombinant full length protein corresponding to Human YWHAE aa 2-254. (With tag; BC000179).
DDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKN VIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDIL DVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVA YKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDD AIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVE DENQ
Database link: P62258
- Mouse brain tissue lysate; HT29, 293T and HeLa cell lysates; Human colon cancer tissue.
This product was previously labelled as 14-3-3 epsilon
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/25 - 1/150.|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Sequence similaritiesBelongs to the 14-3-3 family.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 E antibody
- 14 3 3 epsilon antibody
- 14 3 3E antibody
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue lableing YWHAE with ab182656 at 1/10 dilution (left panel). Right panel is treated with the immunogen.
All lanes : Anti-YWHAE antibody (ab182656) at 1/250 dilution
Lane 1 : HT29 cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Donkey
anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 1 second
10% SDS PAGE
ab182656 has not yet been referenced specifically in any publications.