Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3918] to YWHAE
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-YWHAE antibody [EPR3918]
See all YWHAE primary antibodies
DescriptionRabbit monoclonal [EPR3918] to YWHAE
Tested applicationsSuitable for: ICC/IF, WB, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human YWHAE aa 50-150.
Database link: P62258
- Human fetal brain, SH-SY5Y, 293T and SW480 cell lysates and HeLa cells
This product was previously labelled as 14-3-3 epsilon
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
|Flow Cyt||1/10 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Sequence similaritiesBelongs to the 14-3-3 family.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 E antibody
- 14 3 3 epsilon antibody
- 14 3 3E antibody
All lanes : Anti-YWHAE antibody [EPR3918] (ab92311) at 1/1000 dilution
Lane 1 : Human fetal brain cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 :
293T cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Overlay histogram showing SH-SY5Y cells stained with ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab92311 at 1/100 dilution staining YWHAE in HeLa cells by Immunofluorescence.
ab92311 has been referenced in 4 publications.
- Gao K et al. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. J Cell Sci 128:992-1000 (2015). WB ; Human . PubMed: 25588844
- Zhu Y et al. Neuroprotective effect of TAT-14-3-3e fusion protein against cerebral ischemia/reperfusion injury in rats. PLoS One 9:e93334 (2014). WB ; Rat . PubMed: 24671253
- Hoyo NL et al. Functional EF-hands in neuronal calcium sensor GCAP2 determine its phosphorylation state and subcellular distribution in vivo, and are essential for photoreceptor cell integrity. PLoS Genet 10:e1004480 (2014). WB, IHC-Fr ; Mouse . PubMed: 25058152
- Serrano I et al. Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase. Nat Commun 4:2976 (2013). WB ; Human . PubMed: 24356468