Key features and details
- Rabbit polyclonal to YY1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-YY1 antibody
See all YY1 primary antibodies
DescriptionRabbit polyclonal to YY1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment within Human YY1 (internal sequence). The exact sequence is proprietary.
Database link: P25490
- WB: HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 45 kDa.|
|IP||1/100 - 1/500.|
FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Sequence similaritiesBelongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.
Cellular localizationNucleus matrix. Associated with the nuclear matrix.
- Information by UniProt
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
Anti-YY1 antibody (ab227269) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
YY1 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 μg ab227269. Western blot was performed from the immunoprecipitate using ab227269 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 50 μg (Input).
Lane 2: Control IgG IP in HeLa whole cell lysate.
Lane 3: ab227269 IP in HeLa whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227269 has not yet been referenced specifically in any publications.